Ludwig, Nicole, Leidinger, Petra, Becker, Kurt, Backes, Christina, Fehlmann, Tobias, Pallasch, Christian, Rheinheimer, Steffi, Meder, Benjamin, Staehler, Cord, Meese, Eckart and Keller, Andreas (2016). Distribution of miRNA expression across human tissues. Nucleic Acids Res., 44 (8). S. 3865 - 3878. OXFORD: OXFORD UNIV PRESS. ISSN 1362-4962

Full text not available from this repository.

Abstract

We present a human miRNA tissue atlas by determining the abundance of 1997 miRNAs in 61 tissue biopsies of different organs from two individuals collected post-mortem. One thousand three hundred sixty-four miRNAs were discovered in at least one tissue, 143 were present in each tissue. To define the distribution of miRNAs, we utilized a tissue specificity index (TSI). The majority of miRNAs (82.9%) fell in a middle TSI range i.e. were neither specific for single tissues (TSI > 0.85) nor housekeeping miRNAs (TSI < 0.5). Nonetheless, we observed many different miRNAs and miRNA families that were predominantly expressed in certain tissues. Clustering of miRNA abundances revealed that tissues like several areas of the brain clustered together. Considering -3p and -5p mature forms we observed miR-150 with different tissue specificity. Analysis of additional lung and prostate biopsies indicated that inter-organism variability was significantly lower than inter-organ variability. Tissue-specific differences between the miRNA patterns appeared not to be significantly altered by storage as shown for heart and lung tissue. MiRNAs TSI values of human tissues were significantly (P = 10(-8)) correlated with those of rats; miRNAs that were highly abundant in certain human tissues were likewise abundant in according rat tissues.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Ludwig, NicoleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Leidinger, PetraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Becker, KurtUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Backes, ChristinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fehlmann, TobiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pallasch, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rheinheimer, SteffiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meder, BenjaminUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Staehler, CordUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meese, EckartUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Keller, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-275933
DOI: 10.1093/nar/gkw116
Journal or Publication Title: Nucleic Acids Res.
Volume: 44
Number: 8
Page Range: S. 3865 - 3878
Date: 2016
Publisher: OXFORD UNIV PRESS
Place of Publication: OXFORD
ISSN: 1362-4962
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CIRCULATING MICRORNAS; RNA; BIOMARKERS; PROFILES; ATLAS; BLOOD; GENE; HEPATITIS; CARCINOMA; DISEASEMultiple languages
Biochemistry & Molecular BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/27593

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item