Imhof, Thomas, Rosenblatt, Katharina, Pryymachuk, Galyna, Weiland, Daniela, Noetzel, Nicolas ORCID: 0000-0002-1826-1001, Deschner, James, Baris, Olivier R., Kimoloi, Sammy, Koch, Manuel, Wiesner, Rudolf J. and Korkmaz, Yueksel (2020). Epithelial loss of mitochondrial oxidative phosphorylation leads to disturbed enamel and impaired dentin matrix formation in postnatal developed mouse incisor. Sci Rep, 10 (1). BERLIN: NATURE RESEARCH. ISSN 2045-2322

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Abstract

The formation of dentin and enamel matrix depends on reciprocal interactions between epithelial-mesenchymal cells. To assess the role of mitochondrial function in amelogenesis and dentinogenesis, we studied postnatal incisor development in K320E-Twinkle(Epi) mice. In these mice, a loss of mitochondrial DNA (mtDNA), followed by a severe defect in the oxidative phosphorylation system is induced specifically in Keratin 14 (K14+) expressing epithelial cells. Histochemical staining showed severe reduction of cytochrome c oxidase activity only in K14+epithelial cells. In mutant incisors, H&E staining showed severe defects in the ameloblasts, in the epithelial cells of the stratum intermedium and the papillary cell layer, but also a disturbed odontoblast layer. The lack of amelogenin in the enamel matrix of K320E-Twinkle(Epi) mice indicated that defective ameloblasts are not able to form extracellular enamel matrix proteins. In comparison to control incisors, von Kossa staining showed enamel biomineralization defects and dentin matrix impairment. In mutant incisor, TUNEL staining and ultrastructural analyses revealed differentiation defects, while in hair follicle cells apoptosis is prevalent. We concluded that mitochondrial oxidative phosphorylation in epithelial cells of the developed incisor is required for Ca2+homeostasis to regulate the formation of enamel matrix and induce the differentiation of ectomesenchymal cells into odontoblasts.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Imhof, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rosenblatt, KatharinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pryymachuk, GalynaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weiland, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Noetzel, NicolasUNSPECIFIEDorcid.org/0000-0002-1826-1001UNSPECIFIED
Deschner, JamesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Baris, Olivier R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kimoloi, SammyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koch, ManuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wiesner, Rudolf J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Korkmaz, YuekselUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-307871
DOI: 10.1038/s41598-020-77954-7
Journal or Publication Title: Sci Rep
Volume: 10
Number: 1
Date: 2020
Publisher: NATURE RESEARCH
Place of Publication: BERLIN
ISSN: 2045-2322
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GENE SPLICE PRODUCTS; ORGAN DEVELOPMENT; STEM-CELLS; AMELOGENIN; MORPHOGENESIS; TRANSPORT; DIFFERENTIATION; AMELOBLASTS; APOPTOSIS; NECROSISMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/30787

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