Universität zu Köln

Scicluna, Brendon P., Uhel, Fabrice, van Vught, Lonneke A., Wiewel, Maryse A., Hoogendijk, Arie J., Baessman, Ingelore, Franitza, Marek, Nuernberg, Peter, Horn, Janneke, Cremer, Olaf L., Bonten, Marc J., Schultz, Marcus J. and van der Poll, Tom (2020). The leukocyte non-coding RNA landscape in critically ill patients with sepsis. eLife, 9. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD. ISSN 2050-084X

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Abstract

The extent of non-coding RNA alterations in patients with sepsis and their relationship to clinical characteristics, soluble mediators of the host response to infection, as well as an advocated in vivo model of acute systemic inflammation is unknown. Here we obtained whole blood from 156 patients with sepsis and 82 healthy subjects among whom eight were challenged with lipopolysaccharide in a clinically controlled setting (human endotoxemia). Via next-generation microarray analysis of leukocyte RNA we found that long non-coding RNA and, to a lesser extent, small non-coding RNA were significantly altered in sepsis relative to health. Long non-coding RNA expression, but not small non-coding RNA, was largely recapitulated in human endotoxemia. Integrating RNA profiles and plasma protein levels revealed known as well as previously unobserved pathways, including non-sensory olfactory receptor activity. We provide a benchmark dissection of the blood leukocyte 'regulome' that can facilitate prioritization of future functional studies.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Scicluna, Brendon P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Uhel, Fabrice UNSPECIFIED UNSPECIFIED UNSPECIFIED van Vught, Lonneke A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiewel, Maryse A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoogendijk, Arie J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Baessman, Ingelore UNSPECIFIED UNSPECIFIED UNSPECIFIED Franitza, Marek UNSPECIFIED UNSPECIFIED UNSPECIFIED Nuernberg, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Horn, Janneke UNSPECIFIED UNSPECIFIED UNSPECIFIED Cremer, Olaf L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonten, Marc J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schultz, Marcus J. UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Poll, Tom UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-308190
DOI:	10.7554/eLife.58597
Journal or Publication Title:	eLife
Volume:	9
Date:	2020
Publisher:	ELIFE SCIENCES PUBLICATIONS LTD
Place of Publication:	CAMBRIDGE
ISSN:	2050-084X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTENSIVE-CARE-UNIT; TRANSCRIPTIONAL LANDSCAPE; OLFACTORY RECEPTORS; ACQUIRED PNEUMONIA; GENE-EXPRESSION; HOST RESPONSE; MORTALITY; MOUSE; TOLERANCE; SIGNATURE Multiple languages Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/30819