Universität zu Köln

Reder, Henrike, Taferner, Victor F., Wittekindt, Claus, Braeuninger, Andreas, Speel, Ernst-Jan M., Gattenloehner, Stefan, Wolf, Gregor, Klussmann, Jens P., Wuerdemann, Nora and Wagner, Steffen (2020). Plasma Cell-Free Human Papillomavirus Oncogene E6 and E7 DNA Predicts Outcome in Oropharyngeal Squamous Cell Carcinoma. J. Mol. Diagn., 22 (11). S. 1333 - 1344. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1943-7811

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Abstract

Persistent human papillomavirus (HPV) infection is associated with the development of oropharyngeal squamous cell carcinoma (OPSCC), and increasing incidences of OPSCC are reported. The generally favorable treatment outcome in patients with HPV-driven OPSCC has brought de-escalation of treatment into discussion. Nevertheless, 13% to 25% develop a relapse within two years after current standard treatment. New biomarkers are urgently required to monitor therapy response, tumor burden, and minimal residual disease during follow-up. This observational study examined 50 patients with OPSCC to investigate plasma cell-free (cf) HPV-DNA derived from tumor cells before therapy and during follow-up. Real-time quantitative PCR was applied to quantify the DNA concentration of HPV oncogenes E6 and E7. A total of 85.7% of pretreatment samples from patients with HPV-driven OPSCC (n = 28) were positive for at least one marker, and cfHPV-DNA concentration increased with tumor size. Virtually no signals were detected in HPV-negative OPSCC patients (n = 20; P <= 0.001). Patients without clinical evidence of recurrence had significantly reduced cfHPV-DNA concentrations after therapy (P <= 0.001). Conversely, cfHPV-DNA levels increased or remained above threshold in five patients who had residual disease or developed recurrence. In conclusion, plasma cfHPV-DNA detection correlates with the clinical course of disease in patients with HPV-driven OPSCC. Consequently, extensive clinical investigation should be considered if cfHPV-DNA is detected during follow-up of patients with HPV-driven OPSCC.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Reder, Henrike UNSPECIFIED UNSPECIFIED UNSPECIFIED Taferner, Victor F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wittekindt, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED Braeuninger, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Speel, Ernst-Jan M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gattenloehner, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Wolf, Gregor UNSPECIFIED UNSPECIFIED UNSPECIFIED Klussmann, Jens P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wuerdemann, Nora UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagner, Steffen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-313800
DOI:	10.1016/j.jmoldx.2020.08.002
Journal or Publication Title:	J. Mol. Diagn.
Volume:	22
Number:	11
Page Range:	S. 1333 - 1344
Date:	2020
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1943-7811
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BARR-VIRUS DNA; NUCLEIC-ACIDS; HPV-DNA; HEAD; P16(INK4A); EXPRESSION; RECURRENT; SURVIVAL; NSCLC; P16 Multiple languages Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31380