Universität zu Köln

Tzaridis, Theophilos, Reiners, Katrin S., Weller, Johannes, Bachurski, Daniel ORCID: 0000-0001-9168-9680, Schaefer, Niklas, Schaub, Christina, Hallek, Michael, Scheffler, Bjoern, Glas, Martin, Herrlinger, Ulrich, Wild, Stefan, Coch, Christoph and Hartmann, Gunther (2020). Analysis of Serum miRNA in Glioblastoma Patients: CD44-Based Enrichment of Extracellular Vesicles Enhances Specificity for the Prognostic Signature. Int. J. Mol. Sci., 21 (19). BASEL: MDPI. ISSN 1422-0067

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Abstract

Glioblastoma is a devastating disease, for which biomarkers allowing a prediction of prognosis are urgently needed. microRNAs have been described as potentially valuable biomarkers in cancer. Here, we studied a panel of microRNAs in extracellular vesicles (EVs) from the serum of glioblastoma patients and evaluated their correlation with the prognosis of these patients. The levels of 15 microRNAs in EVs that were separated by size-exclusion chromatography were studied by quantitative real-time PCR, followed by CD44 immunoprecipitation (SEC + CD44), and compared with those from the total serum of glioblastoma patients (n = 55) and healthy volunteers (n = 10). Compared to total serum, we found evidence for the enrichment of miR-21-3p and miR-106a-5p and, conversely, lower levels of miR-15b-3p, in SEC + CD44 EVs. miR-15b-3p and miR-21-3p were upregulated in glioblastoma patients compared to healthy subjects. A significant correlation with survival of the patients was found for levels of miR-15b-3p in total serum and miR-15b-3p, miR-21-3p, miR-106a-5p, and miR-328-3p in SEC + CD44 EVs. Combining miR-15b-3p in serum or miR-106a-5p in SEC + CD44 EVs with any one of the other three microRNAs in SEC + CD44 EVs allowed for a prognostic stratification of glioblastoma patients. We have thus identified four microRNAs in glioblastoma patients whose levels, in combination, can predict the prognosis for these patients.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tzaridis, Theophilos UNSPECIFIED UNSPECIFIED UNSPECIFIED Reiners, Katrin S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Weller, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Bachurski, Daniel UNSPECIFIED orcid.org/0000-0001-9168-9680 UNSPECIFIED Schaefer, Niklas UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaub, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Hallek, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Scheffler, Bjoern UNSPECIFIED UNSPECIFIED UNSPECIFIED Glas, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Herrlinger, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Wild, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Coch, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Gunther UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-316266
DOI:	10.3390/ijms21197211
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	21
Number:	19
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MICRORNA EXPRESSION PROFILE; PROGRESSION-FREE SURVIVAL; RNA; GLIOMA; TEMOZOLOMIDE; MECHANISM; THERAPY Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31626