Universität zu Köln

Masangkay, Frederick R., Milanez, Giovanni D., Somsak, Voravuth, Kotepui, Manas, Tangpong, Jitbanjong and Karanis, Panagiotis (2020). Multi-spatial contamination of environmental aquatic matrices withCryptosporidium: a climate, health, and regulatory framework for the Philippines. Environ. Sci Eur., 32 (1). NEW YORK: SPRINGER. ISSN 2190-4715

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Abstract

Background Cryptosporidiumis a waterborne global pathogen causing diarrhea primarily in infants and immunocompromised individuals. The Philippines is a tropical country susceptible to the influences of climate change and water crises. To date, the country has no existing epidemiologic data, regulation, or strategy for monitoringCryptosporidiumin freshwater systems. We, therefore, endeavored to provide evidence on the multi-spatial contamination ofCryptosporidiumin environmental aquatic matrices using low-cost, user-friendly, and sustainable strategies and submit implications on the presence ofCryptosporidiumin freshwater systems in a climate, health, and regulatory framework. Results Here, we present the microscopic detection ofCryptosporidiumoocysts in low-volume (50 mL) environmental samples of surface water (SW), sediments (BW), and substrate-associated biofilm (SAB) and in 1 L bulk SW investigated by PCR. The multi-spatial distribution ofCryptosporidiumoocysts in the low-volume (50 ml) aquatic matrices based on microscopy was highest at 69% (20/29) in SW and lowest at 50% (13/26) in BW. Immunofluorescence technique provided the highest microscopic positivity rate with 59% (17/29), 38% (10/26), and 50% (10/20) detection in SW, BW, and SAB, respectively. The detection and identification ofCryptosporidiumin 1 L bulk SW by PCR and sequence analysis was recorded in total at 21% (6/29) in sampling sites where the differential identification ofC. parvum, C. hominis,andCryptosporidiumspp. was 7% (2/29), 10% (3/29), and 3% (1/29), respectively. Conclusions We report the microscopical and first molecular epidemiologic data ofCryptosporidiumfrom the most significant environmental freshwater systems in the Philippines. The presence of the two main human and animal pathogenic speciesC. parvumandC. hominisfrom the largest lakes and major water reservoirs in the country calls for sustainable solutions in safeguarding the quality of freshwater resources in a climate, health, and regulatory approach.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Masangkay, Frederick R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Milanez, Giovanni D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Somsak, Voravuth UNSPECIFIED UNSPECIFIED UNSPECIFIED Kotepui, Manas UNSPECIFIED UNSPECIFIED UNSPECIFIED Tangpong, Jitbanjong UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-318305
DOI:	10.1186/s12302-020-00410-w
Journal or Publication Title:	Environ. Sci Eur.
Volume:	32
Number:	1
Date:	2020
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	2190-4715
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CRYPTOSPORIDIUM-PARVUM OOCYSTS; DRINKING-WATER; WORLDWIDE OUTBREAKS; GIARDIA-DUODENALIS; SURFACE-WATER; BIOFILMS; HOKKAIDO; RIVER; SPP.; RAW Multiple languages Environmental Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31830