Kreer, Christoph, Zehner, Matthias, Weber, Timm, Ercanoglu, Meryem S., Gieselmann, Lutz, Rohde, Cornelius, Halwe, Sandro, Korenkov, Michael, Schommers, Philipp ORCID: 0000-0003-3375-6800, Vanshylla, Kanika, Di Cristanziano, Veronica, Janicki, Hanna, Brinker, Reinhild, Ashurov, Artem, Kraehling, Verena, Kupke, Alexandra, Cohen-Dvashi, Hadas, Koch, Manuel, Eckert, Jan Mathis, Lederer, Simone, Pfeifer, Nico, Wolf, Timo, Vehreschild, Maria J. G. T., Wendtner, Clemens, Diskin, Ron, Gruell, Henning, Becker, Stephan and Klein, Florian (2020). Longitudinal Isolation of Potent Near-Germline SARS-CoV-2-Neutralizing Antibodies from COVID-19 Patients. Cell, 182 (4). S. 843 - 867. CAMBRIDGE: CELL PRESS. ISSN 1097-4172

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Abstract

The SARS-CoV-2 pandemic has unprecedented implications for public health, social life, and the world economy. Because approved drugs and vaccines are limited or not available, new options for COVID-19 treatment and prevention are in high demand. To identify SARS-CoV-2-neutralizing antibodies, we analyzed the antibody response of 12 COVID-19 patients from 8 to 69 days after diagnosis. By screening 4,313 SARS-CoV-2-reactive B cells, we isolated 255 antibodies from different time points as early as 8 days after diagnosis. Of these, 28 potently neutralized authentic SARS-CoV-2 with IC100 as low as 0.04 mu g/mL, showing a broad spectrum of variable (V) genes and low levels of somatic mutations. Interestingly, potential precursor sequences were identified in naive B cell repertoires from 48 healthy individuals who were sampled before the COVID-19 pandemic. Our results demonstrate that SARS-CoV-2-neutralizing antibodies are readily generated from a diverse pool of precursors, fostering hope for rapid induction of a protective immune response upon vaccination.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kreer, ChristophUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zehner, MatthiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weber, TimmUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ercanoglu, Meryem S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gieselmann, LutzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rohde, CorneliusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Halwe, SandroUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Korenkov, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schommers, PhilippUNSPECIFIEDorcid.org/0000-0003-3375-6800UNSPECIFIED
Vanshylla, KanikaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Di Cristanziano, VeronicaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Janicki, HannaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brinker, ReinhildUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ashurov, ArtemUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kraehling, VerenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kupke, AlexandraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cohen-Dvashi, HadasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koch, ManuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eckert, Jan MathisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lederer, SimoneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pfeifer, NicoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wolf, TimoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vehreschild, Maria J. G. T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wendtner, ClemensUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Diskin, RonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gruell, HenningUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Becker, StephanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Klein, FlorianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-322909
DOI: 10.1016/j.cell.2020.06.044
Journal or Publication Title: Cell
Volume: 182
Number: 4
Page Range: S. 843 - 867
Date: 2020
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 1097-4172
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
NEUTRALIZING ANTIBODIES; MONOCLONAL-ANTIBODIES; B-CELLS; VACCINE; HIV; BROAD; GENERATION; SEQUENCE; DESIGNMultiple languages
Biochemistry & Molecular Biology; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/32290

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