Universität zu Köln

Piper, Thomas, Fusshoeller, Gregor, Geyer, Hans, Toboc, Ani, Danila, Madalin-George and Thevis, Mario (2020). Detecting the misuse of 7-oxo-DHEA by means of carbon isotope ratio mass spectrometry in doping control analysis. Rapid Commun. Mass Spectrom., 34 (12). HOBOKEN: WILEY. ISSN 1097-0231

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Abstract

Rationale The misuse of 7-oxo-DHEA (3 beta-hydroxyandrost-5-ene-7,17-dione) is prohibited according to the World Anti-Doping Agency (WADA) code. Nevertheless, it is easily available as a dietary supplement and from black market sources. In two recent doping control samples, significant amounts of its main metabolite 7 beta-OH-DHEA were identified, necessitating further investigations. Methods As both 7-oxo-DHEA and 7 beta-OH-DHEA are endogenously produced steroids and no concentration thresholds applicable to routine doping controls exist, the development and validation of a carbon isotope ratio (CIR) mass spectrometry method ha been desirable. Excretion studies encompassing 7-oxo-DHEA, 7-oxo-DHEA-acetate, and in-house deuterated 7-oxo-DHEA were conducted and evaluated with regard to urinary CIR and potential new metabolites of 7-oxo-DHEA. Results Numerous urinary metabolites were identified, some of which have not been reported before, while others corroborate earlier findings on the metabolism of 7-oxo-DHEA. The CIRs of both 7-oxo-DHEA and 7 beta-OH-DHEA were significantly influenced for more than 50 h after a single oral dose of 100 mg, and a novel metabolite (5 alpha-androstane-3 beta,7 beta-diol-17-one) was found to prolong this detection time window by approximately 25 h. Applying the validated method to routine doping control specimens presenting atypically high urinary 7 beta-OH-DHEA levels clearly demonstrated the exogenous origin of 7-oxo-DHEA and 7 beta-OH-DHEA. Conclusions As established for other endogenously produced steroids such as testosterone, the CIR allows for a clear differentiation between endo- and exogenous sources of 7-oxo-DHEA and 7 beta-OH-DHEA. The novel metabolites detected after administration may help to improve the detection of 7-oxo-DHEA misuse and simplify its detection in doping control specimens.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Piper, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Fusshoeller, Gregor UNSPECIFIED UNSPECIFIED UNSPECIFIED Geyer, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Toboc, Ani UNSPECIFIED UNSPECIFIED UNSPECIFIED Danila, Madalin-George UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-328875
DOI:	10.1002/rcm.8776
Journal or Publication Title:	Rapid Commun. Mass Spectrom.
Volume:	34
Number:	12
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1097-0231
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOGENOUS URINARY STEROIDS; REFERENCE-POPULATION; GAS-CHROMATOGRAPHY; C-13/C-12 RATIOS; FRACTIONATION Multiple languages Biochemical Research Methods; Chemistry, Analytical; Spectroscopy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/32887