Universität zu Köln

Masangkay, Frederick R., Milanez, Giovanni D., Tsiami, Amalia, Hapan, Freida Z., Somsak, Voravuth, Kotepui, Manas, Tangpong, Jitbanjong and Karanis, Panagiotis (2020). Waterborne protozoan pathogens in environmental aquatic biofilms: Implications for water quality assessment strategies. Environ. Pollut., 259. OXFORD: ELSEVIER SCI LTD. ISSN 1873-6424

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Abstract

Biofilms containing pathogenic organisms from the water supply are a potential source of protozoan parasite outbreaks and a significant public health concern. The aim of the present study was to demonstrate the simultaneous and multi-spatial occurrence of waterborne protozoan pathogens (WBPP) in substrate-associated biofilms (SAB) and compare it to surface water (SW) and sediments with bottom water (BW) counterparts using manual filtration and elution from low-volume samples. For scenario purposes, simulated environmental biofilm contamination was created from in-situ grown one-month-old SAB (OM-SAB) that were spiked with Cryptosporidium parvum oocysts. Samples were collected from the largest freshwater reservoirs in Luzon, Philippines and a University Lake in Thailand. A total of 69 samples (23 SAB, 23 SW, and 23 BW) were evaluated using traditional staining techniques for Cryptosporidium, and Immunofluorescence staining for the simultaneous detection of Cryptosporidium and Giardia. WBPP were found in 43% SAB, 39% SW, and 39% BW of the samples tested in the present study with SAB results reflecting SW and BW results. Further highlights were demonstrated in the potential of using low-volume samples for the detection of parasites in source water. Scanning electron microscopy of OM-SAB samples revealed a naturally-associated testate amoeba shell, while Cryptosporidium oocysts spiked samples provided a visual profile of what can be expected from naturally contaminated biofilms. This study provides the first evidence for the simultaneous and multi-spatial occurrence of waterborne protozoan pathogens in low-volume aquatic matrices and further warrants SAB testing along with SW and BW matrices for improved water quality assessment strategies (iWQAS). (C) 2020 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Masangkay, Frederick R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Milanez, Giovanni D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tsiami, Amalia UNSPECIFIED UNSPECIFIED UNSPECIFIED Hapan, Freida Z. UNSPECIFIED UNSPECIFIED UNSPECIFIED Somsak, Voravuth UNSPECIFIED UNSPECIFIED UNSPECIFIED Kotepui, Manas UNSPECIFIED UNSPECIFIED UNSPECIFIED Tangpong, Jitbanjong UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-338421
DOI:	10.1016/j.envpol.2019.113903
Journal or Publication Title:	Environ. Pollut.
Volume:	259
Date:	2020
Publisher:	ELSEVIER SCI LTD
Place of Publication:	OXFORD
ISSN:	1873-6424
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CRYPTOSPORIDIUM-PARVUM OOCYSTS; DRINKING-WATER; WORLDWIDE OUTBREAKS; GIARDIA-DUODENALIS; TOXOPLASMA-GONDII; RIVER WATER; WASTE-WATER; NESTED PCR; TRANSMISSION; SAMPLES Multiple languages Environmental Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33842