Universität zu Köln

Paterno, Giuseppe Maria ORCID: 0000-0003-2349-566X, Colombo, Elisabetta, Vurro, Vito, Lodola, Francesco ORCID: 0000-0002-3506-5619, Cimo, Simone ORCID: 0000-0003-0333-5386, Sesti, Valentina ORCID: 0000-0003-1277-644X, Molotokaite, Egle, Bramini, Mattia ORCID: 0000-0002-0381-9391, Ganzer, Lucia, Fazzi, Daniele ORCID: 0000-0002-8515-4214, D'Andrea, Cosimo, Benfenati, Fabio, Bertarelli, Chiara and Lanzani, Guglielmo (2020). Membrane Environment Enables Ultrafast Isomerization of Amphiphilic Azobenzene. Adv. Sci., 7 (8). HOBOKEN: WILEY. ISSN 2198-3844

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Abstract

The non-covalent affinity of photoresponsive molecules to biotargets represents an attractive tool for achieving effective cell photo-stimulation. Here, an amphiphilic azobenzene that preferentially dwells within the plasma membrane is studied. In particular, its isomerization dynamics in different media is investigated. It is found that in molecular aggregates formed in water, the isomerization reaction is hindered, while radiative deactivation is favored. However, once protected by a lipid shell, the photochromic molecule reacquires its ultrafast photoisomerization capacity. This behavior is explained considering collective excited states that may form in aggregates, locking the conformational dynamics and redistributing the oscillator strength. By applying the pump probe technique in different media, an isomerization time in the order of 10 ps is identified and the deactivation in the aggregate in water is also characterized. Finally, it is demonstrated that the reversible modulation of membrane potential of HEK293 cells via illumination with visible light can be indeed related to the recovered trans -> cis photoreaction in lipid membrane. These data fully account for the recently reported experiments in neurons, showing that the amphiphilic azobenzenes, once partitioned in the cell membrane, are effective light actuators for the modification of the electrical state of the membrane.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Paterno, Giuseppe Maria UNSPECIFIED orcid.org/0000-0003-2349-566X UNSPECIFIED Colombo, Elisabetta UNSPECIFIED UNSPECIFIED UNSPECIFIED Vurro, Vito UNSPECIFIED UNSPECIFIED UNSPECIFIED Lodola, Francesco UNSPECIFIED orcid.org/0000-0002-3506-5619 UNSPECIFIED Cimo, Simone UNSPECIFIED orcid.org/0000-0003-0333-5386 UNSPECIFIED Sesti, Valentina UNSPECIFIED orcid.org/0000-0003-1277-644X UNSPECIFIED Molotokaite, Egle UNSPECIFIED UNSPECIFIED UNSPECIFIED Bramini, Mattia UNSPECIFIED orcid.org/0000-0002-0381-9391 UNSPECIFIED Ganzer, Lucia UNSPECIFIED UNSPECIFIED UNSPECIFIED Fazzi, Daniele UNSPECIFIED orcid.org/0000-0002-8515-4214 UNSPECIFIED D'Andrea, Cosimo UNSPECIFIED UNSPECIFIED UNSPECIFIED Benfenati, Fabio UNSPECIFIED UNSPECIFIED UNSPECIFIED Bertarelli, Chiara UNSPECIFIED UNSPECIFIED UNSPECIFIED Lanzani, Guglielmo UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-338563
DOI:	10.1002/advs.201903241
Journal or Publication Title:	Adv. Sci.
Volume:	7
Number:	8
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	2198-3844
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Physical Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PHOTOISOMERIZATION; PERMEABILITY; SURFACTANT; MIXTURES; VESICLES Multiple languages Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/33856