Universität zu Köln

Li, Jiahui, Betzler, Christopher, Lohneis, Philipp, Popp, Marie Christine, Qin, Jiwei, Kalinski, Thomas, Wartmann, Thomas, Bruns, Christiane J., Zhao, Yue ORCID: 0000-0002-6790-3402 and Popp, Felix C. (2020). The IL-17A/IL-17RA Axis Is Not Related to Overall Survival and Cancer Stem Cell Modulation in Pancreatic Cancer. Int. J. Mol. Sci., 21 (6). BASEL: MDPI. ISSN 1422-0067

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Abstract

(1) Background: IL-17A accelerates pancreatic intraepithelial neoplasia (PanIN) progression. In this study, we examined whether IL-17A/IL-17RA promotes pancreatic ductal adenocarcinoma (PDAC) aggressiveness in terms of survival and cancer stem cell modulation. (2) Methods: In vitro, the wound-healing assay, the sphere formation assay, and flow cytometry were applied to assess cancer stem cell features. In vivo, pancreatic tumors were induced in C57BL/6 mice using electroporation with oncogenic plasmids (P53-/- R172H; KrasG12V). Anti-IL-17 antibodies were administered as immunotherapy. We analyzed IL-17A/IL-17RA related survival using publicly available transcriptomic data (n = 903). (3) Results: IL-17A/IL-17RA expression was not related to survival in PDAC patients. IL-17A neither induces stem cell markers nor increases sphere formation and cell motility in vitro. Blocking the IL-17A/IL-17RA axis in a murine pancreatic cancer model did not improve the survival of mice, but reduced the tumor burden slightly. (4) Conclusions: IL-17A does not promote stem cell expansion in PDAC cell lines. Blocking IL-17A/IL-17RA signaling does not interfere with pancreatic cancer development and progression and may not be considered as a promising monotherapy for PDAC.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Li, Jiahui UNSPECIFIED UNSPECIFIED UNSPECIFIED Betzler, Christopher UNSPECIFIED UNSPECIFIED UNSPECIFIED Lohneis, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Popp, Marie Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Qin, Jiwei UNSPECIFIED UNSPECIFIED UNSPECIFIED Kalinski, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Wartmann, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Bruns, Christiane J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhao, Yue UNSPECIFIED orcid.org/0000-0002-6790-3402 UNSPECIFIED Popp, Felix C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-342038
DOI:	10.3390/ijms21062215
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	21
Number:	6
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34203