Universität zu Köln

Lass, Anna ORCID: 0000-0002-5885-9868, Ma, Liqing, Kontogeorgos, Ioannis, Li, Xiuping and Karanis, Panagiotis (2020). Contamination of wastewater with Echinococcus multilocularis - possible implications for drinking water resources in the QTP China. Water Res., 170. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 0043-1354

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Abstract

Echinococcus multilocularis is a parasite that causes a dangerous zoonosis, alveolar echinococcosis (AE). Its presence in water sources, however, has scarcely been studied heretofore. Accordingly, 222 samples of different origin including wastewater from wastewater treatment plants (WWTPs) (n = 137), slaugh-terhouse (n = 49) as well as water from rivers (n = 26) and a cattle farm (n = 10) were collected from Xining City and a rural area in Qinghai-Tibet Plateau (QTP), an endemic area. Material obtained after processing of 10 L volume samples was subsequently analysed using three molecular detection methods: nested PCR, real-time PCR and LAMP. E. multiloculans DNA was found in 13 (5.85%) water samples; including 8 (5.8%), 3 (6%), 2 (20%) and 0 positive samples found in WWTPs, a slaughterhouse, a cattle farm and rivers, respectively. All three (LAMP, PCR, RT-PCR) molecular tools displayed high agreement and effectiveness in their ability of detecting the parasite's DNA in environmental material. This is the first investigation describing E. multilocularis detection in wastewater samples, using three sensitive molecular diagnostic tools. Results indicate the role of wastewater in dissemination of E. multilocularis and the risk of contamination of water sources. (C) 2019 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lass, Anna UNSPECIFIED orcid.org/0000-0002-5885-9868 UNSPECIFIED Ma, Liqing UNSPECIFIED UNSPECIFIED UNSPECIFIED Kontogeorgos, Ioannis UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Xiuping UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-343647
DOI:	10.1016/j.watres.2019.115334
Journal or Publication Title:	Water Res.
Volume:	170
Date:	2020
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	0043-1354
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN ALVEOLAR ECHINOCOCCOSIS; HUMAN CYSTIC ECHINOCOCCOSIS; RISK-FACTORS; 1ST DETECTION; EGGS; CRYPTOSPORIDIUM; IDENTIFICATION; PROVINCE; PCR; DNA Multiple languages Engineering, Environmental; Environmental Sciences; Water Resources Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34364