Universität zu Köln

Wang, Mengying, Isachenko, Evgenia, Todorov, Plamen, Rahimi, Gohar, Mallmann, Peter, Katkov, Igor I. and Isachenko, Vladimir ORCID: 0000-0002-3674-543X (2020). Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability. Biomed Res. Int., 2020. LONDON: HINDAWI LTD. ISSN 2314-6141

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Abstract

This study aimed to compare the quality of human spermatozoa vitrified by direct plunging into liquid nitrogen vs. liquid air. Spermatozoa were divided into three groups: fresh spermatozoa (Group F) were used as a control. Spermatozoa suspension (20 mu l) was vitrified in open granules by direct dropping into liquid nitrogen (Group LN) or clean liquid air (Group LA). After warming at 37 degrees C, the progressive motility rate of Group F was reduced from 65.9 +/- 2.5% to 34.0 +/- 1.9% (Group LN) and 38.1 +/- 2.3% (Group LA), respectively (P-1-2,P-3 < 0.05). The reductions in viability were 65.6 +/- 2.2%, 29.0 +/- 1.8%, and 36.6 +/- 2.6% for Groups F, LN, and LA, respectively (P-1-2,P-3 < 0.05). Comparing spermatozoa vitrified in liquid nitrogen vs. liquid air, no significant differences were detected in motility (34.0 +/- 1.9% vs. 38.1 +/- 2.3%), viability (29.0 +/- 1.8% vs. 36.6 +/- 2.6%), early apoptosis (13.8 +/- 1.5% vs. 14.3 +/- 1.8%), late apoptosis (45.5 +/- 1.8% vs. 43.7 +/- 2.2%), and necrosis (19.5 +/- 2.0% vs. 15.0 +/- 1.8%; p>0.01 for all respective differences). There was a statistical tendency for increasing rates of progressive motility and viability and decreasing rates of apoptosis and necrosis when comparing spermatozoa vitrified in liquid air vs. liquid nitrogen. It is concluded that cryoprotectant-free vitrification by the direct dropping of human spermatozoa in a clean cooling agent (liquid air) is a good alternative to the use of nonsterile liquid nitrogen and can be used to cool cells while minimising the risk of microbial contamination.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wang, Mengying UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Todorov, Plamen UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahimi, Gohar UNSPECIFIED UNSPECIFIED UNSPECIFIED Mallmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Katkov, Igor I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Vladimir UNSPECIFIED orcid.org/0000-0002-3674-543X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-348276
DOI:	10.1155/2020/2934315
Journal or Publication Title:	Biomed Res. Int.
Volume:	2020
Date:	2020
Publisher:	HINDAWI LTD
Place of Publication:	LONDON
ISSN:	2314-6141
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MICROBIAL-CONTAMINATION; CROSS-CONTAMINATION; CRYOPRESERVATION; EMBRYOS; SPERM; SEMEN; STORAGE; MEN; NITROGEN; BANKING Multiple languages Biotechnology & Applied Microbiology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34827