Universität zu Köln

Walther, Matthias, Schrahn, Sandy, Krauss, Veiko, Lein, Sandro, Kessler, Jeannette, Jenuwein, Thomas and Reuter, Gunter (2020). Heterochromatin formation in Drosophila requires genome-wide histone deacetylation in cleavage chromatin before mid-blastula transition in early embryogenesis. Chromosoma, 129 (1). S. 83 - 99. NEW YORK: SPRINGER. ISSN 1432-0886

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Abstract

Su(var) mutations define epigenetic factors controlling heterochromatin formation and gene silencing in Drosophila. Here, we identify SU(VAR)2-1 as a novel chromatin regulator that directs global histone deacetylation during the transition of cleavage chromatin into somatic blastoderm chromatin in early embryogenesis. SU(VAR)2-1 is heterochromatin-associated in blastoderm nuclei but not in later stages of development. In larval polytene chromosomes, SU(VAR)2-1 is a band-specific protein. SU(VAR)2-1 directs global histone deacetylation by recruiting the histone deacetylase RPD3. In Su(var)2-1 mutants H3K9, H3K27, H4K8 and H4K16 acetylation shows elevated levels genome-wide and heterochromatin displays aberrant histone hyper-acetylation. Whereas H3K9me2- and HP1a-binding appears unaltered, the heterochromatin-specific H3K9me2S10ph composite mark is impaired in heterochromatic chromocenters of larval salivary polytene chromosomes. SU(VAR)2-1 contains an NRF1/EWG domain and a C2HC zinc-finger motif. Our study identifies SU(VAR)2-1 as a dosage-dependent, heterochromatin-initiating SU(VAR) factor, where the SU(VAR)2-1-mediated control of genome-wide histone deacetylation after cleavage and before mid-blastula transition (pre-MBT) is required to enable heterochromatin formation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Walther, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Schrahn, Sandy UNSPECIFIED UNSPECIFIED UNSPECIFIED Krauss, Veiko UNSPECIFIED UNSPECIFIED UNSPECIFIED Lein, Sandro UNSPECIFIED UNSPECIFIED UNSPECIFIED Kessler, Jeannette UNSPECIFIED UNSPECIFIED UNSPECIFIED Jenuwein, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Reuter, Gunter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-348721
DOI:	10.1007/s00412-020-00732-x
Journal or Publication Title:	Chromosoma
Volume:	129
Number:	1
Page Range:	S. 83 - 99
Date:	2020
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-0886
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POSITION-EFFECT VARIEGATION; DNA METHYLATION; GENE ENCODES; TRANSCRIPTION; MUTATIONS; PROTEIN; MELANOGASTER; SUPPRESSION; CELLS; SU(VAR)3-9 Multiple languages Biochemistry & Molecular Biology; Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34872