Universität zu Köln

Rinschen, Markus M., Benzing, Thomas, Limbutara, Kavee and Pisitkun, Trairak (2015). Proteomic analysis of the kidney filtration barrier-Problems and perspectives. Proteom. Clin. Appl., 9 (11-12). S. 1053 - 1069. WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1862-8354

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Abstract

Diseases of the glomerular filter of the kidney are a leading cause of end-stage renal failure. The kidney filter is localized within the renal glomeruli, small microvascular units that are responsible for ultrafiltration of about 180 liters of primary urine every day. The renal filter consists of three layers, fenestrated endothelial cells, glomerular basement membrane, and the podocytes, terminally differentiated, arborized epithelial cells. This review demonstrates the use of proteomics to generate insights into the regulation of the renal filtration barrier at a molecular level. The advantages and disadvantages of different glomerular purification methods are examined, and the technical limitations that have been significantly improved by in silico or biochemical approaches are presented. We also comment on phosphoproteomic studies that have generated considerable molecular-level understanding of the physiological regulation of the kidney filter. Lastly, we conclude with an analysis of urinary exosomes as a potential filter-derived resource for the noninvasive discovery of glomerular disease mechanisms.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rinschen, Markus M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Benzing, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Limbutara, Kavee UNSPECIFIED UNSPECIFIED UNSPECIFIED Pisitkun, Trairak UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-384864
DOI:	10.1002/prca.201400201
Journal or Publication Title:	Proteom. Clin. Appl.
Volume:	9
Number:	11-12
Page Range:	S. 1053 - 1069
Date:	2015
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1862-8354
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language 2-DIMENSIONAL GEL-ELECTROPHORESIS; LASER CAPTURE MICRODISSECTION; RESISTANT NEPHROTIC SYNDROME; PROTEIN EXPRESSION PROFILE; LARGE-SCALE IDENTIFICATION; RAT MESANGIAL CELLS; SLIT DIAPHRAGM; MASS-SPECTROMETRY; HUMAN URINE; PHOSPHOPROTEOMIC ANALYSIS Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38486