Universität zu Köln

Recks, Mascha S., Grether, Nicolai B., van der Broeck, Franziska, Ganscher, Alla, Wagner, Nicole, Henke, Erik ORCID: 0000-0003-2380-2682, Erguen, Sueleyman, Schroeter, Michael and Kuerten, Stefanie ORCID: 0000-0001-6119-605X (2015). Four different synthetic peptides of proteolipid protein induce a distinct antibody response in MP4-induced experimental autoimmune encephalomyelitis. Clin. Immunol., 159 (1). S. 93 - 107. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1521-7035

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Abstract

Here we studied the autoantibody specificity elicited by proteolipid protein (PLP) in MP4-induced experimental autoimmune encephalomyelitis, a mouse model of multiple sclerosis (MS). In C57BL/6 (B6) mice, antibodies were induced by immunization with one of the two extracellular and by the intracellular PLP domain. Antibodies against extracellular PLP were myelin-reactive in oligodendrocyte cultures and induced mild spinal cord demyelination upon transfer into B cell-deficient J(H)T mice. Remarkably, also antibodies against intracellular PLP showed binding to intact oligodendrocytes and were capable of inducing myelin pathology upon transfer into J(H)T mice. In MP4-immunized mice peptide-specific T(H)1/T(H)17 responses were mainly directed against the extracellular PLP domains, but also involved the intracellular epitopes. These data suggest that both extracellular and intracellular epitopes of PLP contribute to the pathogenesis of MP4-induced EAE already in the setting of intact myelin. It remains to be elucidated if this concept also applies to MS itself. (C) 2015 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Recks, Mascha S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Grether, Nicolai B. UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Broeck, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Ganscher, Alla UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagner, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Henke, Erik UNSPECIFIED orcid.org/0000-0003-2380-2682 UNSPECIFIED Erguen, Sueleyman UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeter, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuerten, Stefanie UNSPECIFIED orcid.org/0000-0001-6119-605X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-400222
DOI:	10.1016/j.clim.2015.04.020
Journal or Publication Title:	Clin. Immunol.
Volume:	159
Number:	1
Page Range:	S. 93 - 107
Date:	2015
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1521-7035
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; B-CELL; MULTIPLE-SCLEROSIS; FUSION PROTEIN; EXTRACELLULAR DOMAIN; MYELINATED FIBERS; G-RATIO; ANTIGEN; PENETRATION; PATHOLOGY Multiple languages Immunology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40022