Universität zu Köln

Schild, Marius, Ruhs, Aaron, Beiter, Thomas ORCID: 0000-0001-7299-7303, Zuegel, Martina, Hudemann, Jens, Reimer, Anna, Krumholz-Wagner, Ilke, Wagner, Carola, Keller, Janine, Eder, Klaus, Krueger, Karsten, Krueger, Marcus ORCID: 0000-0003-2008-4582, Braun, Thomas ORCID: 0000-0002-6165-4804, Niess, Andreas, Steinacker, Juergen and Mooren, Frank C. (2015). Basal and exercise induced label-free quantitative protein profiling of m. vastus lateralis in trained and untrained individuals. J. Proteomics, 122. S. 119 - 133. AMSTERDAM: ELSEVIER. ISSN 1876-7737

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Abstract

Morphological and metabolic adaptations of the human skeletal muscle to exercise are crucial to improve performance and prevent chronic diseases and metabolic disorders. In this study we investigated human skeletal muscle protein composition in endurance trained (ET) versus untrained individuals (lit) and its modulation by an acute bout of endurance exercise. Participants were recruited based on their VO(2)max and subjected to a bicycle exercise test. M. vastus lateralis biopsies were taken before and three hours after exercise. Muscle lysates were analyzed using off-gel LC-MS/MS. Relative protein abundances were compared between ET and UT at rest and after exercise. Comparing UT and ET, we identified 92 significantly changed proteins under resting conditions. Specifically, fiber-type-specific and proteins of the oxidative phosphorylation and tricarboxylic acid cycle were increased in ET. In response to acute exercise, 71 proteins in ET and 44 in UT were altered. Here, a decrease of proteins involved in energy metabolism accompanied with alterations of heat shock and proteasomal proteins could be observed. In summary, long-term endurance training increased the basal level of structural and mitochondrial proteins in skeletal muscle. In contrast, acute exercise resulted in a depletion of proteins related to substrate utilization, especially in trained athletes. Biological significance The investigation of the human skeletal muscle proteome in response to exercise may provide novel insights into the process of muscular plasticity. It is of importance in the development of exercise-based strategies in the prevention and therapy of many chronic inflammatory and degenerative diseases which are often accompanied by muscular deconditioning. Up to date, proteomic investigations of the human muscle proteome in adaptation to exercise are mainly focused on untrained individuals and often restricted to animal studies. In the present study we compare the protein composition in endurance trained athletes and untrained individuals in the resting muscle and its modulation in response to acute exercise. To our knowledge, we present the first comprehensive analysis of skeletal muscle proteome alterations in response to acute and long-term exercise intervention. (C) 2015 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schild, Marius UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruhs, Aaron UNSPECIFIED UNSPECIFIED UNSPECIFIED Beiter, Thomas UNSPECIFIED orcid.org/0000-0001-7299-7303 UNSPECIFIED Zuegel, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Hudemann, Jens UNSPECIFIED UNSPECIFIED UNSPECIFIED Reimer, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Krumholz-Wagner, Ilke UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagner, Carola UNSPECIFIED UNSPECIFIED UNSPECIFIED Keller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Eder, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Karsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Marcus UNSPECIFIED orcid.org/0000-0003-2008-4582 UNSPECIFIED Braun, Thomas UNSPECIFIED orcid.org/0000-0002-6165-4804 UNSPECIFIED Niess, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Steinacker, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Mooren, Frank C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-401281
DOI:	10.1016/j.jprot.2015.03.028
Journal or Publication Title:	J. Proteomics
Volume:	122
Page Range:	S. 119 - 133
Date:	2015
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1876-7737
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OXIDATIVE ENZYME-ACTIVITY; SKELETAL-MUSCLE PROTEOME; HIGH-INTENSITY EXERCISE; FIBER TYPES; MITOCHONDRIAL; ADAPTATIONS; KINASE; COMPARTMENTATION; SUPPLEMENTATION; METABOLISM Multiple languages Biochemical Research Methods Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/40128