Universität zu Köln

Merino, O., Aguagueina, W. E., Esponda, P., Risopatron, J., Isachenko, E., Isachenko, V. and Sanchez, R. (2015). Protective effect of butylated hydroxytoluene on sperm function in human spermatozoa cryopreserved by vitrification technique. Andrologia, 47 (2). S. 186 - 194. HOBOKEN: WILEY. ISSN 1439-0272

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Abstract

Butylhydroxytoluene (BHT), a synthetic analogue of vitamin E, shows antioxidant and antiviral properties and has been successfully used for mammalian sperm cryopreservation. In this study, BHT was included in a vitrification solution to determine its cryoprotective effect on human spermatozoa. Spermatozoa were selected by swim-up and vitrified in close sealed straw using either a combination of human tubal fluid (HTF), sucrose and BHT 1mm (VMBHT), or only HTF and sucrose (VM). The optimal concentration of BHT was determined by the observation of preserved progressive sperm motility (PSM) after warming and detection of plasma membrane (PMI), membrane mitochondrial potential (m) and DNA integrity. The presence of reactive oxygen species (ROS) was also detected. The PSM was significantly higher in the VMBHT group (80.86 +/- 5.41%) compared with the VM group (68.9 +/- 3.67%) (P<0.05). Butylhydroxytoluene significantly preserved DNA integrity (4.0 +/- 0.1% versus 6.1 +/- 1.6%; P<0.05) and reduced ROS production (5.5 +/- 2.2 versus 8.6 +/- 1.8%; P<0.05). Plasma membrane and m showed no statistical differences. One millimolar BHT effectively maintained cell function and due to its antioxidant and antiviral properties could be used in semen cryopreservation of patients with viral infections transmitted by seminal plasma.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Merino, O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Aguagueina, W. E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Esponda, P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Risopatron, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sanchez, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-412069
DOI:	10.1111/and.12246
Journal or Publication Title:	Andrologia
Volume:	47
Number:	2
Page Range:	S. 186 - 194
Date:	2015
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1439-0272
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN-IMMUNODEFICIENCY-VIRUS; BOAR SPERMATOZOA; SEMEN; CRYOPROTECTANTS; TRANSMISSION; FERTILITY; VIABILITY; CAPTURE; STRESS; FROZEN Multiple languages Andrology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41206