Universität zu Köln

Effertz, Christoph, Mueller, Stefan and von Elert, Eric ORCID: 0000-0001-7758-716X (2015). Differential Peptide Labeling (iTRAQ) in LC-MS/MS Based Proteomics in Daphnia Reveal Mechanisms of an Antipredator Response. J. Proteome Res., 14 (2). S. 888 - 897. WASHINGTON: AMER CHEMICAL SOC. ISSN 1535-3907

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Abstract

Daphnia, an important model organism for studies on ecology and evolution, has become a textbook example for inducible defenses against predators. Inducible defenses are widespread in nature, and the underlying molecular mechanisms for this plasticity in general and in particular in Daphnia are not fully understood. Here, we provide for the first time a combination of established life-history changes (LHC), which are induced by chemical cues of a predator (fish kairomones), in Daphnia with differential peptide labeling (iTRAQ) in LC-MS/MS based proteomics. The aim of the present study is the elucidation of proteins involved in specific antipredator responses in a predator-prey system of ecological relevance by high-throughput proteomics. To obtain a highly specific antifish response of Daphnia, highly purified fish kairomones were applied in the presence or absence of light. We were able to identify a set of functional proteins, which are likely to explain the kairomone-mediated and light-dependent LHC in Daphnia.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Effertz, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED von Elert, Eric UNSPECIFIED orcid.org/0000-0001-7758-716X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-413273
DOI:	10.1021/pr500948a
Journal or Publication Title:	J. Proteome Res.
Volume:	14
Number:	2
Page Range:	S. 888 - 897
Date:	2015
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1535-3907
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SEROTONIN REUPTAKE INHIBITORS; LIVER SULFITE OXIDASE; LIFE-HISTORY SHIFTS; VERTICAL MIGRATION; MESSENGER-RNA; BODY-SIZE; PROTEIN; MAGNA; PREDATOR; METABOLISM Multiple languages Biochemical Research Methods Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41327