Jung, Benjamin, Ludewig, Frank, Schulz, Alexander, Meissner, Garvin, Woestefeld, Nicole, Fluegge, Ulf-Ingo, Pommerrenig, Benjamin ORCID: 0000-0002-7522-7942, Wirsching, Petra, Sauer, Norbert, Koch, Wolfgang, Sommer, Frederik, Muehlhaus, Timo, Schroda, Michael ORCID: 0000-0001-6872-0483, Cuin, Tracey Ann, Graus, Dorothea, Marten, Irene, Hedrich, Rainer ORCID: 0000-0003-3224-1362 and Neuhaus, H. Ekkehard (2015). Identification of the transporter responsible for sucrose accumulation in sugar beet taproots. Nat. Plants, 1 (1). LONDON: NATURE PUBLISHING GROUP. ISSN 2055-0278

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Abstract

Sugar beet provides around one third of the sugar consumed worldwide and serves as a significant source of bioenergy in the form of ethanol. Sucrose accounts for up to 18% of plant fresh weight in sugar beet. Most of the sucrose is concentrated in the taproot, where it accumulates in the vacuoles. Despite 30 years of intensive research, the transporter that facilitates taproot sucrose accumulation has escaped identification. Here, we combine proteomic analyses of the taproot vacuolar membrane, the tonoplast, with electrophysiological analyses to show that the transporter BvTST2.1 is responsible for vacuolar sucrose uptake in sugar beet taproots. We show that BvTST2.1 is a sucrose-specific transporter, and present evidence to suggest that it operates as a proton antiporter, coupling the import of sucrose into the vacuole to the export of protons. BvTST2.1 exhibits a high amino acid sequence similarity to members of the tonoplast monosaccharide transporter family in Arabidopsis, prompting us to rename this group of proteins 'tonoplast sugar transporters'. The identification of BvTST2.1 could help to increase sugar yields from sugar beet and other sugar-storing plants in future breeding programs.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Jung, BenjaminUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ludewig, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schulz, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meissner, GarvinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Woestefeld, NicoleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fluegge, Ulf-IngoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pommerrenig, BenjaminUNSPECIFIEDorcid.org/0000-0002-7522-7942UNSPECIFIED
Wirsching, PetraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sauer, NorbertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koch, WolfgangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sommer, FrederikUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Muehlhaus, TimoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schroda, MichaelUNSPECIFIEDorcid.org/0000-0001-6872-0483UNSPECIFIED
Cuin, Tracey AnnUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Graus, DorotheaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Marten, IreneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hedrich, RainerUNSPECIFIEDorcid.org/0000-0003-3224-1362UNSPECIFIED
Neuhaus, H. EkkehardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-414753
DOI: 10.1038/NPLANTS.2014.1
Journal or Publication Title: Nat. Plants
Volume: 1
Number: 1
Date: 2015
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2055-0278
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
MONOSACCHARIDE TRANSPORTER; VACUOLAR MEMBRANE; ARABIDOPSIS; TONOPLAST; STORAGE; PROTEINS; TISSUE; PHOSPHORYLATION; GERMINATION; GROWTHMultiple languages
Plant SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/41475

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