Universität zu Köln

Weber, Stefanie, Hofmann, Andrea, Herms, Stefan ORCID: 0000-0002-2786-8200, Hoffmann, Per and Doerfler, Walter (2015). Destabilization of the human epigenome: consequences of foreign DNA insertions. Epigenomics, 7 (5). S. 745 - 756. LONDON: FUTURE MEDICINE LTD. ISSN 1750-192X

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Abstract

Aim: We previously reported changes of DNA methylation and transcription patterns in mammalian cells that carry integrated foreign DNA. Experiments were now designed to assess the epigenetic consequences of inserting a 5.6 kbp plasmid into the human genome. Methods: Differential transcription and CpG methylation patterns were compared between transgenomic and nontransgenomic cell clones by using gene chip microarray systems. Results: In 4.7% of the 28.869 gene segments analyzed, transcriptional activities were up-or downregulated in the transgenomic cell clones. Genome-wide profiling revealed differential methylation in 3791 of >480,000 CpG's examined in transgenomic versus nontransgenomic clones. Conclusion: The data document genome-wide effects of foreign DNA insertions on the epigenetic stability of human cells. Many fields in experimental biology and medicine employ transgenomic or otherwise genome-manipulated cells or organisms without considering the epigenetic consequences for the recipient genomes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Weber, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Herms, Stefan UNSPECIFIED orcid.org/0000-0002-2786-8200 UNSPECIFIED Hoffmann, Per UNSPECIFIED UNSPECIFIED UNSPECIFIED Doerfler, Walter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-415819
DOI:	10.2217/EPI.15.40
Journal or Publication Title:	Epigenomics
Volume:	7
Number:	5
Page Range:	S. 745 - 756
Date:	2015
Publisher:	FUTURE MEDICINE LTD
Place of Publication:	LONDON
ISSN:	1750-192X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DE-NOVO METHYLATION; RECIPIENT GENOMES; MAMMALIAN GENOME; HAMSTER-CELLS; INTEGRATION; SEQUENCES; ADENOVIRUS-TYPE-12; NORMALIZATION; DISCOVERY; PATTERNS Multiple languages Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41581