Isachenko, V., Isachenko, E., Sanchez, R., Dattena, M., Mallmann, P. and Rahimi, G. (2015). Cryopreservation of Whole Ovine Ovaries with Pedicles as a Model for Human: Parameters of Perfusion with Simultaneous Saturations by Cryoprotectants. Clin. Lab., 61 (3-4). S. 415 - 421. HEIDELBERG: CLIN LAB PUBL. ISSN 1433-6510

Full text not available from this repository.

Abstract

Background: The aim of this research was to study the effectiveness of perfusion of intact ovine ovaries with different rates of perfusion and time-period elapsed between extraction of these ovaries and the beginning of perfusion. Methods: Ovaries were perfused through the arteria ovarica (ovarian arteries) with culture medium supplemented with 5% bovine calf serum, 6% dimethyl sulfoxide, 6% ethylene glycol, 0.15M sucrose, Indian ink, and 100 IU/mL heparin at room temperature (22 degrees C). In the first cycle of experiments, ovaries (n = 96) were perfused for 60 minutes just after extraction of ovaries at the following rates of perfusion (mL/hour): 150, 100, 75, 50, 25, 12.5, and 6.3. In the second cycle of experiments, ovaries (n = 26) were perfused at a rate of 25 mL/hour for 60 minutes after extraction of ovaries and their storage at room temperature for 2, 3, 4, and 5 hours, for groups 1, 2, 3, and, 4, respectively. Successful perfusion of blood vessels was detected visible by a blue coloration of the ovarian tissues. Results: The first cycle of experiments showed that the optimal perfusion rates were 50 mL/hour and 25 mL/hour. In the second cycle of experiments, good perfusion of ovaries was established at a perfusion rate of 25 mL/hour for the ovaries of groups when 2 and 3 hours had elapsed after extraction. Conclusions: Effective perfusion of ovine intact ovaries with vascular pedicle was established using freezing medium at room temperature at the rate of perfusion of 25 mL/hour or 50 mL/hour. The ovaries must be perfused not later than 3 hours after the death of animals.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Isachenko, V.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Isachenko, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sanchez, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dattena, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mallmann, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rahimi, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-416768
DOI: 10.7754/Clin.Lab.2014.140919
Journal or Publication Title: Clin. Lab.
Volume: 61
Number: 3-4
Page Range: S. 415 - 421
Date: 2015
Publisher: CLIN LAB PUBL
Place of Publication: HEIDELBERG
ISSN: 1433-6510
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SHEEP OVARIES; FERTILITY PRESERVATION; INTACT OVARY; NUDE-MICE; TRANSPLANTATION; TISSUE; AUTOTRANSPLANTATION; VIABILITY; VITRIFICATION; AUTOGRAFTSMultiple languages
Medical Laboratory TechnologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/41676

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item