Universität zu Köln

Alcazar, Ruben ORCID: 0000-0002-3567-7586, von Reth, Marcel, Bautor, Jaqueline, Chae, Eunyoung ORCID: 0000-0002-0889-9837, Weigel, Detlef ORCID: 0000-0002-2114-7963, Koornneef, Maarten ORCID: 0000-0002-7759-4869 and Parker, Jane E. (2014). Analysis of a Plant Complex Resistance Gene Locus Underlying Immune-Related Hybrid Incompatibility and Its Occurrence in Nature. PLoS Genet., 10 (12). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1553-7404

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Abstract

Mechanisms underlying speciation in plants include detrimental (incompatible) genetic interactions between parental alleles that incur a fitness cost in hybrids. We reported on recessive hybrid incompatibility between an Arabidopsis thaliana strain from Poland, Landsberg erecta (Ler), and many Central Asian A. thaliana strains. The incompatible interaction is determined by a polymorphic cluster of Toll/interleukin-1 receptor-nucleotide binding-leucine rich repeat (TNL) RPP1 (Recognition of Peronospora parasitica1)-like genes in Ler and alleles of the receptor-like kinase Strubbelig Receptor Family 3 (SRF3) in Central Asian strains Kas-2 or Kond, causing temperature-dependent autoimmunity and loss of growth and reproductive fitness. Here, we genetically dissected the RPP1-like Ler locus to determine contributions of individual RPP1-like Ler (R1-R8) genes to the incompatibility. In a neutral background, expression of most RPP1-like Ler genes, except R3, has no effect on growth or pathogen resistance. Incompatibility involves increased R3 expression and engineered R3 overexpression in a neutral background induces dwarfism and sterility. However, no individual RPP1-like Ler gene is sufficient for incompatibility between Ler and Kas-2 or Kond, suggesting that co-action of at least two RPP1-like members underlies this epistatic interaction. We find that the RPP1-like Ler haplotype is frequent and occurs with other Ler RPP1-like alleles in a local population in Gorzow Wielkopolski (Poland). Only Gorzow individuals carrying the RPP1-like Ler haplotype are incompatible with Kas-2 and Kond, whereas other RPP1-like alleles in the population are compatible. Therefore, the RPP1-like Ler haplotype has been maintained in genetically different individuals at a single site, allowing exploration of forces shaping the evolution of RPP1-like genes at local and regional population scales.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Alcazar, Ruben UNSPECIFIED orcid.org/0000-0002-3567-7586 UNSPECIFIED von Reth, Marcel UNSPECIFIED UNSPECIFIED UNSPECIFIED Bautor, Jaqueline UNSPECIFIED UNSPECIFIED UNSPECIFIED Chae, Eunyoung UNSPECIFIED orcid.org/0000-0002-0889-9837 UNSPECIFIED Weigel, Detlef UNSPECIFIED orcid.org/0000-0002-2114-7963 UNSPECIFIED Koornneef, Maarten UNSPECIFIED orcid.org/0000-0002-7759-4869 UNSPECIFIED Parker, Jane E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-422827
DOI:	10.1371/journal.pgen.1004848
Journal or Publication Title:	PLoS Genet.
Volume:	10
Number:	12
Date:	2014
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1553-7404
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS-THALIANA; ANTIBACTERIAL DEFENSE; RECOMBINATION; EVOLUTION; COEVOLUTION; POPULATIONS; DIVERSITY; POLYMORPHISM; SOFTWARE; SEQUENCE Multiple languages Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42282