Universität zu Köln

Peschke, Katrin, Weitzmann, Anke, Heger, Klaus, Behrendt, Rayk ORCID: 0000-0002-1091-2877, Schubert, Nadja, Scholten, Julia, Voehringer, David, Hartmann, Karin ORCID: 0000-0002-4595-8226, Dudeck, Anne ORCID: 0000-0002-1311-9620, Schmidt-Supprian, Marc ORCID: 0000-0002-8543-6166 and Roers, Axel (2014). I kappa B Kinase 2 Is Essential for IgE-Induced Mast Cell De Novo Cytokine Production but Not for Degranulation. Cell Reports, 8 (5). S. 1300 - 1308. CAMBRIDGE: CELL PRESS. ISSN 2211-1247

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Abstract

The immunoglobulin E (IgE)-mediated mast cell (MC) response is central to the pathogenesis of type I allergy and asthma. I kappa B kinase 2 (IKK2) was reported to couple IgE-induced signals to MC degranulation by phosphorylating the SNARE protein SNAP23. We investigated MC responses in mice with MC-specific inactivation of IKK2 or NF-kappa B essential modulator (NEMO), or animals with MC-specific expression of a mutant, constitutively active IKK2. We show that the IgE-induced late-phase cytokine response is reduced in mice lacking IKK2 or NEMO in MCs. However, anaphylactic in vivo responses of these animals are not different from those of control mice, and in vitro IKK2-deficient MCs readily phosphorylate SNAP23 and degranulate similarly to control cells in response to allergen or calcium ionophore. Constitutive overactivation of the NF-kappa B pathway has only slight effects on allergen-triggered MC responses. Thus, IKK2 is dispensable for MC degranulation, and the important question how IgE-induced signals trigger MC vesicle fusion remains open.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Peschke, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Weitzmann, Anke UNSPECIFIED UNSPECIFIED UNSPECIFIED Heger, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Behrendt, Rayk UNSPECIFIED orcid.org/0000-0002-1091-2877 UNSPECIFIED Schubert, Nadja UNSPECIFIED UNSPECIFIED UNSPECIFIED Scholten, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Voehringer, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Karin UNSPECIFIED orcid.org/0000-0002-4595-8226 UNSPECIFIED Dudeck, Anne UNSPECIFIED orcid.org/0000-0002-1311-9620 UNSPECIFIED Schmidt-Supprian, Marc UNSPECIFIED orcid.org/0000-0002-8543-6166 UNSPECIFIED Roers, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-428764
DOI:	10.1016/j.celrep.2014.07.046
Journal or Publication Title:	Cell Reports
Volume:	8
Number:	5
Page Range:	S. 1300 - 1308
Date:	2014
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2211-1247
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NUCLEAR-FACTOR; T-CELLS; ACTIVATION; MICE; INHIBITION; DISEASE Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42876