Universität zu Köln

Frobel, Joana, Hemeda, Hatim, Lenz, Michael ORCID: 0000-0002-6319-6480, Abagnale, Giulio, Joussen, Sylvia, Denecke, Bernd, Saric, Tomo ORCID: 0000-0001-8344-1095, Zenke, Martin and Wagner, Wolfgang ORCID: 0000-0002-1971-3217 (2014). Epigenetic Rejuvenation of Mesenchymal Stromal Cells Derived from Induced Pluripotent Stem Cells. Stem Cell Rep., 3 (3). S. 414 - 423. CAMBRIDGE: CELL PRESS. ISSN 2213-6711

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Abstract

Standardization of mesenchymal stromal cells (MSCs) remains a major obstacle in regenerative medicine. Starting material and culture expansion affect cell preparations and render comparison between studies difficult. In contrast, induced pluripotent stem cells (iPSCs) assimilate toward a ground state and may therefore give rise to more standardized cell preparations. We reprogrammed MSCs into iPSCs, which were subsequently redifferentiated toward MSCs. These iPS-MSCs revealed similar morphology, immunophenotype, in vitro differentiation potential, and gene expression profiles as primary MSCs. However, iPS-MSCs were impaired in suppressing T cell proliferation. DNA methylation (DNAm) profiles of iPSCs maintained donor-specific characteristics, whereas tissue-specific, senescence-associated, and age-related DNAm patterns were erased during reprogramming. iPS-MSCs reacquired senescence-associated DNAm during culture expansion, but they remained rejuvenated with regard to age-related DNAm. Overall, iPS-MSCs are similar to MSCs, but they reveal incomplete reacquisition of immunomodulatory function and MSC-specific DNAm patterns-particularly of DNAm patterns associated with tissue type and aging.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Frobel, Joana UNSPECIFIED UNSPECIFIED UNSPECIFIED Hemeda, Hatim UNSPECIFIED UNSPECIFIED UNSPECIFIED Lenz, Michael UNSPECIFIED orcid.org/0000-0002-6319-6480 UNSPECIFIED Abagnale, Giulio UNSPECIFIED UNSPECIFIED UNSPECIFIED Joussen, Sylvia UNSPECIFIED UNSPECIFIED UNSPECIFIED Denecke, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Saric, Tomo UNSPECIFIED orcid.org/0000-0001-8344-1095 UNSPECIFIED Zenke, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagner, Wolfgang UNSPECIFIED orcid.org/0000-0002-1971-3217 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-428842
DOI:	10.1016/j.stemcr.2014.07.003
Journal or Publication Title:	Stem Cell Rep.
Volume:	3
Number:	3
Page Range:	S. 414 - 423
Date:	2014
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2213-6711
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DNA METHYLATION CHANGES; CPG SITES; SENESCENCE; DONOR Multiple languages Cell & Tissue Engineering; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42884