Universität zu Köln

Parr, Maria Kristina ORCID: 0000-0001-7407-8300, Zhao, Piwen, Haupt, Oliver, Ngueu, Sandrine Tchoukouegno, Hengevoss, Jonas, Fritzemeier, Karl Heinrich, Piechotta, Marion, Schloerer, Nils ORCID: 0000-0002-0990-9582, Muhn, Peter, Zheng, Wen-Ya, Xie, Ming-Yong and Diel, Patrick (2014). Estrogen receptor beta is involved in skeletal muscle hypertrophy induced by the phytoecdysteroid ecdysterone. Mol. Nutr. Food Res., 58 (9). S. 1861 - 1873. HOBOKEN: WILEY. ISSN 1613-4133

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Abstract

Scope: The phytoectysteroid ecdysterone (Ecdy) was reported to stimulate protein synthesis and enhance physical performance. The aim of this study was to investigate underlying molecular mechanisms particularly the role of ER beta (ER beta). Results: In male rats, Ecdy treatment increased muscle fiber size, serum IGF-1 increased, and corticosteron and 17 beta-estradiol (E2) decreased. In differentiated C2C12 myoblastoma cells, treatment with Ecdy, dihydrotestosterone, IGF-1 but also E2 results in hypertrophy. Hypertrophy induced by E2 and Ecdy could be antagonized with an antiestrogen but not by an antiandrogen. In HEK293 cells transfected with ER alpha (ER alpha) or ER beta, Ecdy treatment transactivated a reporter gene. To elucidate the role of ER alpha in Ecdy-mediated muscle hypertrophy, C2C12 myotubes were treated with ER alpha (ALPHA) and ER beta (BETA) selective ligands. Ecdy and BETA treatment but not ALPHA induced hypertrophy. The effect of Ecdy, E2, and BETA could be antagonized by an ER beta-selective antagonist (ANTIBETA). In summary, our results indicate that ER beta is involved in the mediation of the anabolic activity of the Ecdy. Conclusion: These findings provide new therapeutic perspectives for the treatment of muscle injuries, sarcopenia, and cachectic disease, but also imply that such a substance could be abused for doping purposes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Parr, Maria Kristina UNSPECIFIED orcid.org/0000-0001-7407-8300 UNSPECIFIED Zhao, Piwen UNSPECIFIED UNSPECIFIED UNSPECIFIED Haupt, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Ngueu, Sandrine Tchoukouegno UNSPECIFIED UNSPECIFIED UNSPECIFIED Hengevoss, Jonas UNSPECIFIED UNSPECIFIED UNSPECIFIED Fritzemeier, Karl Heinrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Piechotta, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Schloerer, Nils UNSPECIFIED orcid.org/0000-0002-0990-9582 UNSPECIFIED Muhn, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Zheng, Wen-Ya UNSPECIFIED UNSPECIFIED UNSPECIFIED Xie, Ming-Yong UNSPECIFIED UNSPECIFIED UNSPECIFIED Diel, Patrick UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-430049
DOI:	10.1002/mnfr.201300806
Journal or Publication Title:	Mol. Nutr. Food Res.
Volume:	58
Number:	9
Page Range:	S. 1861 - 1873
Date:	2014
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1613-4133
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SELECTIVE LIGANDS; PROTEIN-SYNTHESIS; DIFFERENTIATION; HORMONES; GROWTH; ALPHA; CELLS; ACTIVATION; EXPRESSION Multiple languages Food Science & Technology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43004