Castaings, Loren ORCID: 0000-0001-6830-4527, Bergonzi, Sara, Albani, Maria C. ORCID: 0000-0002-8215-020X, Kemi, Ulla, Savolainen, Outi and Coupland, George ORCID: 0000-0001-6988-4172 (2014). Evolutionary conservation of cold-induced antisense RNAs of FLOWERING LOCUS C in Arabidopsis thaliana perennial relatives. Nat. Commun., 5. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Antisense RNA (asRNA) COOLAIR is expressed at A. thaliana FLOWERING LOCUS C (FLC) in response to winter temperatures. Its contribution to cold-induced silencing of FLC was proposed but its functional and evolutionary significance remain unclear. Here we identify a highly conserved block containing the COOLAIR first exon and core promoter at the 3' end of several FLC orthologues. Furthermore, asRNAs related to COOLAIR are expressed at FLC loci in the perennials A. alpina and A. lyrata, although some splicing variants differ from A. thaliana. Study of the A. alpina orthologue, PERPETUAL FLOWERING 1 (PEP1), demonstrates that AaCOOLAIR is induced each winter of the perennial life cycle. Introduction of PEP1 into A. thaliana reveals that AaCOOLAIR cis-elements confer cold-inducibility in this heterologous species while the difference between PEP1 and FLC mRNA patterns depends on both cis-elements and species-specific trans-acting factors. Thus, expression of COOLAIR is highly conserved, supporting its importance in FLC regulation.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Castaings, LorenUNSPECIFIEDorcid.org/0000-0001-6830-4527UNSPECIFIED
Bergonzi, SaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Albani, Maria C.UNSPECIFIEDorcid.org/0000-0002-8215-020XUNSPECIFIED
Kemi, UllaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Savolainen, OutiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Coupland, GeorgeUNSPECIFIEDorcid.org/0000-0001-6988-4172UNSPECIFIED
URN: urn:nbn:de:hbz:38-434320
DOI: 10.1038/ncomms5457
Journal or Publication Title: Nat. Commun.
Volume: 5
Date: 2014
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
VERNALIZATION-INDUCED REPRESSION; EPIGENETIC MAINTENANCE; FUNCTIONAL ELEMENTS; REGULATORY ELEMENTS; SEQUENCE-ANALYSIS; NONCODING RNA; TIME CONTROL; HISTONE H3; FLC LOCUS; LYSINE 27Multiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/43432

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