Universität zu Köln

Kuhn, Michael ORCID: 0000-0002-2841-872X, Hyman, Anthony A. and Beyer, Andreas ORCID: 0000-0002-3891-2123 (2014). Coiled-Coil Proteins Facilitated the Functional Expansion of the Centrosome. PLoS Comput. Biol., 10 (6). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1553-7358

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Abstract

Repurposing existing proteins for new cellular functions is recognized as a main mechanism of evolutionary innovation, but its role in organelle evolution is unclear. Here, we explore the mechanisms that led to the evolution of the centrosome, an ancestral eukaryotic organelle that expanded its functional repertoire through the course of evolution. We developed a refined sequence alignment technique that is more sensitive to coiled coil proteins, which are abundant in the centrosome. For proteins with high coiled-coil content, our algorithm identified 17% more reciprocal best hits than BLAST. Analyzing 108 eukaryotic genomes, we traced the evolutionary history of centrosome proteins. In order to assess how these proteins formed the centrosome and adopted new functions, we computationally emulated evolution by iteratively removing the most recently evolved proteins from the centrosomal protein interaction network. Coiled-coil proteins that first appeared in the animal-fungi ancestor act as scaffolds and recruit ancestral eukaryotic proteins such as kinases and phosphatases to the centrosome. This process created a signaling hub that is crucial for multicellular development. Our results demonstrate how ancient proteins can be co-opted to different cellular localizations, thereby becoming involved in novel functions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kuhn, Michael UNSPECIFIED orcid.org/0000-0002-2841-872X UNSPECIFIED Hyman, Anthony A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Beyer, Andreas UNSPECIFIED orcid.org/0000-0002-3891-2123 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-436406
DOI:	10.1371/journal.pcbi.1003657
Journal or Publication Title:	PLoS Comput. Biol.
Volume:	10
Number:	6
Date:	2014
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1553-7358
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MICROTUBULE-ORGANIZING CENTERS; EVOLUTIONARY HISTORY; ORTHOLOGOUS GROUPS; C-ELEGANS; DATABASE; ANNOTATION; CENTRIOLE; MULTICELLULARITY; IDENTIFICATION; DUPLICATION Multiple languages Biochemical Research Methods; Mathematical & Computational Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43640