Maettner, R., Sterzik, K., Isachenko, V., Strehler, E., Rahimi, G., Alabart, J. L., Sanchez, R., Mallmann, P. and Isachenko, E. (2014). Quality of human spermatozoa: relationship between high-magnification sperm morphology and DNA integrity. Andrologia, 46 (5). S. 547 - 556. HOBOKEN: WILEY-BLACKWELL. ISSN 1439-0272

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Abstract

The aim of this work is to establish the relationship between the morphology of Intracytoplasmic Morphologically Selected Sperm Injection (IMSI)-selected spermatozoa and their DNA integrity. The 45 ejaculates were randomly distributed into three treatment groups: normozoospermic, oligoasthenozoospermic and oligoasthenotheratozoospermic samples. The evaluation of DNA integrity was performed using the sperm chromatin dispersion test. It was established that DNA integrity of spermatozoa is strongly dependent on ejaculate quality (P<0.05). The count of spermatozoa with nonfragmented DNA in normozoospermic samples was high and independent from IMSI-morphological classes (Class 1 versus Class 3, respectively) (P>0.1). With decreased ejaculate quality, the percentage of spermatozoa with nonfragmented DNA decreased significantly (P<0.05) independent from morphological class. Nevertheless, the rate of IMSI-selected spermatozoa with fragmented DNA within of Class 1 in normozoospermic (Group 1), in oligoasthenozoospermic (Group 2) and in oligoasthenotheratozoospermic (Group 3) samples was 21.1%, 31.8% and 54.1%, respectively. In conclusion, there is a direct relationship between morphological parameters of spermatozoa and their DNA integrity. However, the IMSI technique alone is not enough for the selection of spermatozoa with intact nuclei.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Maettner, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sterzik, K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Isachenko, V.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Strehler, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rahimi, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Alabart, J. L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sanchez, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mallmann, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Isachenko, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-437225
DOI: 10.1111/and.12114
Journal or Publication Title: Andrologia
Volume: 46
Number: 5
Page Range: S. 547 - 556
Date: 2014
Publisher: WILEY-BLACKWELL
Place of Publication: HOBOKEN
ISSN: 1439-0272
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CHROMATIN DISPERSION-TEST; IN-VITRO FERTILIZATION; INTRACYTOPLASMIC SPERM; OXIDATIVE STRESS; MALE-INFERTILITY; MALE-FERTILITY; PREGNANCY RATE; INJECTION; FRAGMENTATION; DAMAGEMultiple languages
AndrologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/43722

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