Universität zu Köln

Geisen, Stefan ORCID: 0000-0003-0734-727X, Kudryavtsev, Alexander ORCID: 0000-0002-3818-3610, Bonkowski, Michael ORCID: 0000-0003-2656-1183 and Smirnov, Alexey ORCID: 0000-0001-5361-8232 (2014). Discrepancy between Species Borders at Morphological and Molecular Levels in the Genus Cochliopodium (Amoebozoa, Himatismenida), with the Description of Cochliopodium plurinucleolum n. sp. Protist, 165 (3). S. 364 - 384. MUNICH: ELSEVIER GMBH. ISSN 1434-4610

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Abstract

Amoebae of the genus Cochliopodium are characterized by a tectum that is a layer of scales covering the dorsal surface of the cell. A combination of scale structure, morphological features and, nowadays, molecular information allows species discrimination. Here we describe a soil species Cochliopodium plurinucleolum n. sp. that besides strong genetic divergence from all currently described species of Cochliopodium differs morphologically by the presence of several peripheral nucleoli in the nucleus. Further, we unambiguously show that the Golgi attachment associated with a dictyosome in Cochliopodium is a cytoplasmic microtubule organizing center (MTOC). Last, we provide detailed morphological and molecular information on the sister clade of C. plurinucleolum, containing C. minus, C. minutoidum, C. pentatrifurcatum and C. megatetrastylus. These species share nearly identical sequences of both, small subunit ribosomal RNA and partial Cox1 genes, and nearly identical structure of the scales. Scales of C. pentatrifurcatum differ, however, strongly from scales of the others while sequences of C. pentatrifurcatum and C. minus are nearly identical These discrepancies urge for future sampling efforts to disentangle species characteristics within Cochliopdium and to investigate morphological and molecular patterns that allow reliable species differentiation. (C) 2014 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Geisen, Stefan UNSPECIFIED orcid.org/0000-0003-0734-727X UNSPECIFIED Kudryavtsev, Alexander UNSPECIFIED orcid.org/0000-0002-3818-3610 UNSPECIFIED Bonkowski, Michael UNSPECIFIED orcid.org/0000-0003-2656-1183 UNSPECIFIED Smirnov, Alexey UNSPECIFIED orcid.org/0000-0001-5361-8232 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-440282
DOI:	10.1016/j.protis.2014.04.002
Journal or Publication Title:	Protist
Volume:	165
Number:	3
Page Range:	S. 364 - 384
Date:	2014
Publisher:	ELSEVIER GMBH
Place of Publication:	MUNICH
ISSN:	1434-4610
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FRESH-WATER; PHYLOGENETIC-RELATIONSHIPS; FINE-STRUCTURE; SP RHIZOPODA; AMEBA; DISCOSEA; MINUS Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44028