Klapoetke, Nathan C., Murata, Yasunobu ORCID: 0000-0001-5757-2497, Kim, Sung Soo ORCID: 0000-0002-3508-0413, Pulver, Stefan R., Birdsey-Benson, Amanda, Cho, Yong Ku ORCID: 0000-0001-5621-521X, Morimoto, Tania K., Chuong, Amy S., Carpenter, Eric J., Tian, Zhijian, Wang, Jun ORCID: 0000-0002-8540-8931, Xie, Yinlong, Yan, Zhixiang, Zhang, Yong, Chow, Brian Y., Surek, Barbara, Melkonian, Michael, Jayaraman, Vivek, Constantine-Paton, Martha, Wong, Gane Ka-Shu and Boyden, Edward S. (2014). Independent optical excitation of distinct neural populations. Nat. Methods, 11 (3). S. 338 - 352. LONDON: NATURE PUBLISHING GROUP. ISSN 1548-7105

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Abstract

Optogenetic tools enable examination of how specific cell types contribute to brain circuit functions. A long-standing question is whether it is possible to independently activate two distinct neural populations in mammalian brain tissue. Such a capability would enable the study of how different synapses or pathways interact to encode information in the brain. Here we describe two channelrhodopsins, Chronos and Chrimson, discovered through sequencing and physiological characterization of opsins from over 100 species of alga. Chrimson's excitation spectrum is red shifted by 45 nm relative to previous channelrhodopsins and can enable experiments in which red light is preferred. We show minimal visual system-mediated behavioral interference when using Chrimson in neurobehavioral studies in Drosophila melanogaster. Chronos has faster kinetics than previous channelrhodopsins yet is effectively more light sensitive. Together these two reagents enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Klapoetke, Nathan C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Murata, YasunobuUNSPECIFIEDorcid.org/0000-0001-5757-2497UNSPECIFIED
Kim, Sung SooUNSPECIFIEDorcid.org/0000-0002-3508-0413UNSPECIFIED
Pulver, Stefan R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Birdsey-Benson, AmandaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cho, Yong KuUNSPECIFIEDorcid.org/0000-0001-5621-521XUNSPECIFIED
Morimoto, Tania K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chuong, Amy S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Carpenter, Eric J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tian, ZhijianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wang, JunUNSPECIFIEDorcid.org/0000-0002-8540-8931UNSPECIFIED
Xie, YinlongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yan, ZhixiangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhang, YongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chow, Brian Y.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Surek, BarbaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Melkonian, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jayaraman, VivekUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Constantine-Paton, MarthaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wong, Gane Ka-ShuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Boyden, Edward S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-445520
DOI: 10.1038/NMETH.2836
Journal or Publication Title: Nat. Methods
Volume: 11
Number: 3
Page Range: S. 338 - 352
Date: 2014
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 1548-7105
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
NEURONAL ACTIVATION; SURFACE EXPRESSION; VISUAL PIGMENTS; ER EXPORT; DROSOPHILA; CHANNELRHODOPSIN; PROTEIN; CELLS; TOOLSMultiple languages
Biochemical Research MethodsMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/44552

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