Universität zu Köln

Reber, Laurent L., Marichal, Thomas, Mukai, Kaori, Kita, Yoshihiro, Tokuoka, Suzumi M., Roers, Axel, Hartmann, Karin ORCID: 0000-0002-4595-8226, Karasuyama, Hajime ORCID: 0000-0003-0689-0836, Nadeau, Kari C., Tsai, Mindy ORCID: 0000-0002-8421-4721 and Galli, Stephen J. (2013). Selective ablation of mast cells or basophils reduces peanut-induced anaphylaxis in mice. J. Allergy Clin. Immunol., 132 (4). S. 881 - 900. NEW YORK: MOSBY-ELSEVIER. ISSN 1097-6825

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Abstract

Background: Studies with c-kit mutant mast cell (MC)-deficient mice and antibody-mediated depletion of basophils suggest that both MCs and basophils can contribute to peanut-induced anaphylaxis (PIA). However, interpretation of data obtained by using such approaches is complicated because c-kit mutant mice have several phenotypic abnormalities in addition to MC deficiency and because basophil-depleting antibodies can also react with MCs. \ Objective: We analyzed (1) the changes in the features of PIA in mice after the selective and inducible ablation of MCs or basophils and (2) the possible importance of effector cells other than MCs and basophils in the PIA response. Methods: Wild-type and various mutant mice were orally sensitized with peanut extract and cholera toxin weekly for 4 weeks and challenged intraperitoneally with peanut extract 2 weeks later. Results: Peanut-challenged, MC-deficient Kit W-sh/W-sh mice had reduced immediate hypothermia, as well as a late-phase decrease in body temperature that was abrogated by antibody-mediated depletion of neutrophils. Diphtheria toxin-mediated selective depletion of MCs or basophils in Mcpt5-Cre; iDTR and Mcpt8 DTR mice, respectively, and treatment of wild-type mice with the basophil-depleting antibody Ba103 significantly reduced peanut-induced hypothermia. Non-c-kit mutant MC-and basophil-deficient Cpa3-Cre; Mcl-1(fl/fl) mice had reduced but still significant responses to peanut. Conclusion: Inducible and selective ablation of MCs or basophils in non-c-kit mutant mice can significantly reduce PIA, but partial responses to peanut can still be observed in the virtual absence of both cell types. The neutrophilia in Kit(W-sh/W-sh) mice might influence the responses of these mice in this PIA model.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Reber, Laurent L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Marichal, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Mukai, Kaori UNSPECIFIED UNSPECIFIED UNSPECIFIED Kita, Yoshihiro UNSPECIFIED UNSPECIFIED UNSPECIFIED Tokuoka, Suzumi M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Roers, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Karin UNSPECIFIED orcid.org/0000-0002-4595-8226 UNSPECIFIED Karasuyama, Hajime UNSPECIFIED orcid.org/0000-0003-0689-0836 UNSPECIFIED Nadeau, Kari C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tsai, Mindy UNSPECIFIED orcid.org/0000-0002-8421-4721 UNSPECIFIED Galli, Stephen J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-474914
DOI:	10.1016/j.jaci.2013.06.008
Journal or Publication Title:	J. Allergy Clin. Immunol.
Volume:	132
Number:	4
Page Range:	S. 881 - 900
Date:	2013
Publisher:	MOSBY-ELSEVIER
Place of Publication:	NEW YORK
ISSN:	1097-6825
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FC-GAMMA RIII; ALLERGIC INFLAMMATION; FOOD ALLERGY; IN-VIVO; MOUSE; NEUTROPHILS; ANTIBODY; DEGRANULATION; PATHOGENESIS; FATALITIES Multiple languages Allergy; Immunology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47491