Universität zu Köln

Zajzon, Katalin, Proels, Felicitas and Heermann, Stephan (2013). Concerted interaction of TGF- and GDNF mediates neuronal differentiation. Neuroreport, 24 (13). S. 704 - 712. PHILADELPHIA: LIPPINCOTT WILLIAMS & WILKINS. ISSN 0959-4965

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Abstract

The glial cell-line derived neurotrophic factor (GDNF) is crucial for ureteric bud morphogenesis, spermatogenesis, and development of the enteric nervous system and is a potent survival factor for various neuronal populations. However, the impact of GDNF, at least on cell survival, was found to depend strongly on the presence of transforming growth factor (TGF-). In this study, we investigate the role of TGF- in GDNF-induced neuronal differentiation. In a cell culture paradigm of N2aGT cells (neuroblastoma cell line), we show that TGF- signaling localizes the GDNF ligand-binding receptor GFRa1 to the cell surface, which is a known mechanism by which TGF- is able to facilitate GDNF signaling. TGF--mediated GDNF signaling slightly elevated the phosphorylation state of Ret, the canonical coreceptor for the GPI-linked (glycosyl-phosphatidylinositol) GFRa1. On the basis of morphological as well as immunocytological data, we finally show that GDNF-mediated neuronal differentiation is intensified when GDNF and TGF- act in concert.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zajzon, Katalin UNSPECIFIED UNSPECIFIED UNSPECIFIED Proels, Felicitas UNSPECIFIED UNSPECIFIED UNSPECIFIED Heermann, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-475969
DOI:	10.1097/WNR.0b013e328363f75c
Journal or Publication Title:	Neuroreport
Volume:	24
Number:	13
Page Range:	S. 704 - 712
Date:	2013
Publisher:	LIPPINCOTT WILLIAMS & WILKINS
Place of Publication:	PHILADELPHIA
ISSN:	0959-4965
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADHESION MOLECULE NCAM; GROWTH-FACTOR-BETA; NEUROTROPHIC FACTOR; CELL-ADHESION; DOPAMINERGIC-NEURONS; TYROSINE KINASE; FAMILY LIGANDS; IN-VITRO; GFR-ALPHA-1; RECEPTOR Multiple languages Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47596