Hess, Cornelius, Thomas, Andreas ORCID: 0000-0003-1199-0743, Thevis, Mario, Stratmann, Bernd, Quester, Wulf, Tschoepe, Diethelm, Madea, Burkhard and Musshoff, Frank (2012). Simultaneous determination and validated quantification of human insulin and its synthetic analogues in human blood serum by immunoaffinity purification and liquid chromatography-mass spectrometry. Anal. Bioanal. Chem., 404 (6-7). S. 1813 - 1823. HEIDELBERG: SPRINGER HEIDELBERG. ISSN 1618-2642

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Abstract

Possible fatal complications of human insulin and its synthetic analogues like hypoglycemia require precise classification and quantitative determination of these drugs both for clinical purposes as well as for forensic toxicologists. A procedure was developed for the identification and quantification of human insulin and different long-acting as well as short-acting synthetic insulins in human blood serum specimens. After an immunoaffinity purification step and separation by liquid chromatography, the insulins were characterized by their five- or sixfold protonated molecule ions and diagnostic product ions. Clinical samples of 207 diabetic and 50 non-diabetic patients after the administration of human insulin or oral antidiabetics and forensic samples were analyzed for human/synthetic insulin concentrations. The method was validated according to international guidelines. Limits of detection of the insulins ranged between 1.3 and 2.8 mu U/ml. Recoveries ranged between 33.2 % and 51.7 %. Precision data was in accordance with international guidelines. Clinical samples showed concentrations of human insulin lower than 301 mu U/ml. Our liquid chromatography tandem mass spectrometry procedure allows unambiguous identification and quantification of the intact human insulin and its intact synthetic analogues HumalogA (R), NovologA (R), ApidraA (R), LantusA (R), and LevemirA (R) in human blood serum in clinical and overdose cases. The assay could be successfully tested in patients with diabetes mellitus on therapy with insulins or oral antidiabetics.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Hess, CorneliusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thomas, AndreasUNSPECIFIEDorcid.org/0000-0003-1199-0743UNSPECIFIED
Thevis, MarioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stratmann, BerndUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Quester, WulfUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tschoepe, DiethelmUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Madea, BurkhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Musshoff, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-481689
DOI: 10.1007/s00216-012-6271-5
Journal or Publication Title: Anal. Bioanal. Chem.
Volume: 404
Number: 6-7
Page Range: S. 1813 - 1823
Date: 2012
Publisher: SPRINGER HEIDELBERG
Place of Publication: HEIDELBERG
ISSN: 1618-2642
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
DOPING CONTROL PURPOSES; AMERICAN ASSOCIATION; SEVERE HYPOGLYCEMIA; DEGRADING ENZYME; HUMAN PLASMA; HUMAN URINE; PROINSULIN; IMMUNOASSAY; DEGRADATION; PRODUCTSMultiple languages
Biochemical Research Methods; Chemistry, AnalyticalMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/48168

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