Universität zu Köln

Lautz, Katja, Damm, Anna, Menning, Maureen, Wenger, Julia, Adam, Alexander C., Zigrino, Paola ORCID: 0000-0002-7470-0064, Kremmer, Elisabeth and Kufer, Thomas A. (2012). NLRP10 enhances Shigella-induced pro-inflammatory responses. Cell Microbiol., 14 (10). S. 1568 - 1584. HOBOKEN: WILEY. ISSN 1462-5822

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Abstract

Members of the NLR family evolved as intracellular sensors for bacterial and viral infection. However, our knowledge on the implication of most of the human NLR proteins in innate immune responses still remains fragmentary. Here we characterized the role of human NLRP10 in bacterial infection. Our data revealed that NLRP10 is a cytoplasmic localized protein that positively contributes to innate immune responses induced by the invasive bacterial pathogen Shigella flexneri. SiRNA-mediated knock-down studies showed that NLRP10 contributes to pro-inflammatory cytokine release triggered by Shigella in epithelial cells and primary dermal fibroblasts, by influencing p38 and NF-?B activation. This effect is dependent on the ATPase activity of NLRP10 and its PYD domain. Mechanistically, NLRP10 interacts with NOD1, a NLR that is pivotally involved in sensing of invasive microbes, and both proteins are recruited to the bacterial entry point at the plasma membrane. Moreover, NLRP10 physically interacts with downstream components of the NOD1 signalling pathway, such as RIP2, TAK1 and NEMO. Taken together, our data revealed a novel role of NLRP10 in innate immune responses towards bacterial infection and suggest that NLRP10 functions as a scaffold for the formation of the NOD1Nodosome.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lautz, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Damm, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Menning, Maureen UNSPECIFIED UNSPECIFIED UNSPECIFIED Wenger, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Adam, Alexander C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zigrino, Paola UNSPECIFIED orcid.org/0000-0002-7470-0064 UNSPECIFIED Kremmer, Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Kufer, Thomas A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481853
DOI:	10.1111/j.1462-5822.2012.01822.x
Journal or Publication Title:	Cell Microbiol.
Volume:	14
Number:	10
Page Range:	S. 1568 - 1584
Date:	2012
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1462-5822
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NOD-LIKE RECEPTOR; NF-KAPPA-B; CELL-DEATH; CONTAINING PROTEIN; GENE-EXPRESSION; ACTIVATION; RECOGNITION; FLEXNERI; DOMAIN; RECRUITMENT Multiple languages Cell Biology; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48185