Beier, A. F. J., Schulz, J. C., Doerr, D., Katsen-Globa, A., Sachinidis, A., Hescheler, J. and Zimmermann, H. (2011). Effective surface-based cryopreservation of human embryonic stem cells by vitrification. Cryobiology, 63 (3). S. 175 - 186. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2392

Full text not available from this repository.

Abstract

Human embryonic stem cells (hESCs) are candidates for many applications in the areas of regenerative medicine, tissue engineering, basic scientific research as well as pharmacology and toxicology. However, use of hESCs is limited by their sensitivity to freezing and thawing procedures. Hence, this emerging science needs new, reliable preservation methods for the long-term storage of large quantities of functional hESCs remaining pluripotent after post-thawing and culturing. Here, we present a highly efficient, surface based vitrification method for the cryopreservation of large numbers of adherent hESC colonies, using modified cell culture substrates. This technique results in much better post-thaw survival rate compared to cryopreservation in suspension and allows a quick and precise handling and storage of the cells, indicating low differentiation rates. (C) 2011 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Beier, A. F. J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schulz, J. C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doerr, D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Katsen-Globa, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sachinidis, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hescheler, J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zimmermann, H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-484267
DOI: 10.1016/j.cryobiol.2011.06.003
Journal or Publication Title: Cryobiology
Volume: 63
Number: 3
Page Range: S. 175 - 186
Date: 2011
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication: SAN DIEGO
ISSN: 1090-2392
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
INTRACELLULAR ICE; PLURIPOTENCY; PRESERVATION; PROPAGATION; VIABILITY; APOPTOSIS; THERAPY; OOCYTES; INTACTMultiple languages
Biology; PhysiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/48426

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item