Pesch, Martina and Huelskamp, Martin (2011). Role of TRIPTYCHON in trichome patterning in Arabidopsis. BMC Plant Biol., 11. LONDON: BMC. ISSN 1471-2229

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Abstract

Background: Trichome patterning in Arabidopsis thaliana is governed by three types of activators, R2R3MYB, bHLH and WD40 proteins, and six R3MYB inhibitors. Among the inhibitors TRIPTYCHON (TRY) seems to fulfill a special function. Its corresponding mutants produce trichome clusters whereas all other inhibitors are involved in trichome density regulation. Results: To better understand the role of TRY in trichome patterning we analyzed its transcriptional regulation. A promoter analysis identified the relevant regulatory region for trichome patterning. This essential region contains a fragment required for a double negative feedback loop such that it mediates the repression of TRY/CPC auto-repression. By transforming single cells of pTRY:GUS lines with p35S:GL1, p35S:GL3 and p35S:TTG1 in the presence or absence of p35S:TRY or p35S:CPC we demonstrate that TRY and CPC can suppress the TRY expression without the transcriptional down regulation of the activators. We further show by promoter/CDS swapping experiments for the R3MYB inhibitors TRY and CPC that the TRY protein has specific properties relevant in the context of both, cluster formation and trichome density. Conclusions: Our identification of a TRY promoter fragment mediating a double negative feedback loop reveals new insight in the regulatory network of the trichome patterning machinery. In addition we show that the auto-repression by TRY can occur without a transcriptional down regulation of the activators, suggesting that the differential complex formation model has a biological significance. Finally we show that the unique role of TRY among the inhibitors is a property of the TRY protein.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Pesch, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huelskamp, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-488845
DOI: 10.1186/1471-2229-11-130
Journal or Publication Title: BMC Plant Biol.
Volume: 11
Date: 2011
Publisher: BMC
Place of Publication: LONDON
ISSN: 1471-2229
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
EPIDERMAL-CELL FATE; FLOWERING TIME GENE; FUNCTIONAL-ANALYSIS; MYB PROTEINS; TRANSCRIPTION; DIFFERENTIATION; CAPRICE; BHLH; EXPRESSION; ENCODESMultiple languages
Plant SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/48884

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