Universität zu Köln

Meek, Andrew T., Kronenberg, Nils M., Morton, Andrew, Liehm, Philipp ORCID: 0000-0002-0147-1141, Murawski, Jan, Dalaka, Eleni ORCID: 0000-0003-3306-7466, Booth, Jonathan H., Powis, Simon J. and Gather, Malte C. (2021). Real-time imaging of cellular forces using optical interference. Nat. Commun., 12 (1). BERLIN: NATURE RESEARCH. ISSN 2041-1723

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Abstract

Important dynamic processes in mechanobiology remain elusive due to a lack of tools to image the small cellular forces at play with sufficient speed and throughput. Here, we introduce a fast, interference-based force imaging method that uses the illumination of an elastic deformable microcavity with two rapidly alternating wavelengths to map forces. We show real-time acquisition and processing of data, obtain images of mechanical activity while scanning across a cell culture, and investigate sub-second fluctuations of the piconewton forces exerted by macrophage podosomes. We also demonstrate force imaging of beating neonatal cardiomyocytes at 100 fps which reveals mechanical aspects of spontaneous oscillatory contraction waves in between the main contraction cycles. These examples illustrate the wider potential of our technique for monitoring cellular forces with high throughput and excellent temporal resolution. Studying dynamic processes in mechanobiology has been challenging due to lack of appropriate tools. Here, the authors present an interference-based method, illuminated via two rapidly alternating wavelengths, which enables real-time mapping of nanoscale forces with sub-second mechanical fluctuations.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Meek, Andrew T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kronenberg, Nils M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Morton, Andrew UNSPECIFIED UNSPECIFIED UNSPECIFIED Liehm, Philipp UNSPECIFIED orcid.org/0000-0002-0147-1141 UNSPECIFIED Murawski, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Dalaka, Eleni UNSPECIFIED orcid.org/0000-0003-3306-7466 UNSPECIFIED Booth, Jonathan H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Powis, Simon J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gather, Malte C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-572968
DOI:	10.1038/s41467-021-23734-4
Journal or Publication Title:	Nat. Commun.
Volume:	12
Number:	1
Date:	2021
Publisher:	NATURE RESEARCH
Place of Publication:	BERLIN
ISSN:	2041-1723
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRACTION FORCE; PHASE-CONTRAST; CARDIOMYOCYTES; MICROSCOPY; PRECISION; RIGIDITY; CELLS Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57296