Universität zu Köln

Zhao, Wenbo ORCID: 0000-0002-3418-9433, Wiedemann, Philip, Woelfel, Eva Maria, Neven, Mona, Peters, Stephanie, Imhof, Thomas, Koch, Manuel ORCID: 0000-0002-2962-7814, Busse, Bjoern, Amling, Michael, Schinke, Thorsten and Yorgan, Timur Alexander (2021). Decreased Trabecular Bone Mass in Col22a1-Deficient Mice. Cells, 10 (11). BASEL: MDPI. ISSN 2073-4409

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Abstract

The bone matrix is constantly remodeled by the coordinated activities of bone-forming osteoblasts and bone-resorbing osteoclasts. Whereas type I collagen is the most abundant bone matrix protein, there are several other proteins present, some of them specifically produced by osteoblasts. In a genome-wide expression screening for osteoblast differentiation markers we have previously identified two collagen-encoding genes with unknown function in bone remodeling. Here we show that one of them, Col22a1, is predominantly expressed in bone, cultured osteoblasts, but not in osteoclasts. Based on this specific expression pattern we generated a Col22a1-deficient mouse model, which was analyzed for skeletal defects by mu CT, undecalcified histology and bone-specific histomorphometry. We observed that Col22a1-deficient mice display trabecular osteopenia, accompanied by significantly increased osteoclast numbers per bone surface. In contrast, cortical bone parameters, osteoblastogenesis or bone formation were unaffected by the absence of Col22a1. Likewise, primary osteoblasts from Col22a1-deficient mice did not display a cell-autonomous defect, and they did not show altered expression of Rankl or Opg, two key regulators of osteoclastogenesis. Taken together, we provide the first evidence for a physiological function of Col22a1 in bone remodeling, although the molecular mechanisms explaining the indirect influence of Col22a1 deficiency on osteoclasts remain to be identified.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zhao, Wenbo UNSPECIFIED orcid.org/0000-0002-3418-9433 UNSPECIFIED Wiedemann, Philip UNSPECIFIED UNSPECIFIED UNSPECIFIED Woelfel, Eva Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Neven, Mona UNSPECIFIED UNSPECIFIED UNSPECIFIED Peters, Stephanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Imhof, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED Busse, Bjoern UNSPECIFIED UNSPECIFIED UNSPECIFIED Amling, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Schinke, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Yorgan, Timur Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-577238
DOI:	10.3390/cells10113020
Journal or Publication Title:	Cells
Volume:	10
Number:	11
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2073-4409
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OSTEOGENESIS IMPERFECTA; MUTATION; INTEGRINS; UPDATE; MATRIX; MODEL; FGF23 Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57723