Universität zu Köln

Vanshylla, Kanika ORCID: 0000-0003-4552-9170, Held, Kathrin ORCID: 0000-0001-7057-6935, Eser, Tabea M., Gruell, Henning, Kleipass, Franziska, Stumpf, Ricarda, Jain, Kanika, Weiland, Daniela, Muench, Jan, Gruettner, Berthold, Geldmacher, Christof and Klein, Florian (2021). CD34T+ Humanized Mouse Model to Study Mucosal HIV-1 Transmission and Prevention. Vaccines, 9 (3). BASEL: MDPI. ISSN 2076-393X

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Abstract

Humanized mice are critical for HIV-1 research, but humanized mice generated from cord blood are inefficient at mucosal HIV-1 transmission. Most mucosal HIV-1 transmission studies in mice require fetal tissue-engraftment, the use of which is highly restricted or prohibited. We present a fetal tissue-independent model called CD34T+ with enhanced human leukocyte levels in the blood and improved T cell homing to the gut-associated lymphoid tissue. CD34T+ mice are highly permissive to intra-rectal HIV-1 infection and also show normal env diversification in vivo despite high viral replication. Moreover, mucosal infection in CD34T+ mice can be prevented by infusion of broadly neutralizing antibodies. CD34T+ mice can be rapidly and easily generated using only cord blood cells and do not require any complicated surgical procedures for the humanization process. Therefore, CD34T+ mice provide a novel platform for mucosal HIV-1 transmission studies as well as rapid in vivo testing of novel prevention molecules against HIV-1.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Vanshylla, Kanika UNSPECIFIED orcid.org/0000-0003-4552-9170 UNSPECIFIED Held, Kathrin UNSPECIFIED orcid.org/0000-0001-7057-6935 UNSPECIFIED Eser, Tabea M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gruell, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Kleipass, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Stumpf, Ricarda UNSPECIFIED UNSPECIFIED UNSPECIFIED Jain, Kanika UNSPECIFIED UNSPECIFIED UNSPECIFIED Weiland, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Muench, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Gruettner, Berthold UNSPECIFIED UNSPECIFIED UNSPECIFIED Geldmacher, Christof UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-586118
DOI:	10.3390/vaccines9030198
Journal or Publication Title:	Vaccines
Volume:	9
Number:	3
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2076-393X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Immunology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58611