Universität zu Köln

Tayem, Raneem, Niemann, Catherin, Pesch, Monika, Morgner, Jessica ORCID: 0000-0001-6374-8639, Niessen, Carien M. ORCID: 0000-0002-4892-9391, Wickstrom, Sara A. and Aumailley, Monique ORCID: 0000-0002-4578-0966 (2021). Laminin 332 Is Indispensable for Homeostatic Epidermal Differentiation Programs. J. Invest. Dermatol., 141 (11). S. 2602 - 2614. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1523-1747

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Abstract

The skin epidermis is attached to the underlying dermis by a laminin 332 (Lm332)-rich basement membrane. Consequently, loss of Lm332 leads to the severe blistering disorder epidermolysis bullosa junctionalis in humans and animals. Owing to the indispensable role of Lm332 in keratinocyte adhesion in vivo, the severity of the disease has limited research into other functions of the protein. We have conditionally disrupted Lm332 expression in basal keratinocytes of adult mice. Although blisters develop along the interfollicular epidermis, hair follicle basal cells provide sufficient anchorage of the epidermis to the dermis, making inducible deletion of the Lama3 gene compatible with life. Loss of Lm332 promoted the thickening of the epidermis and exaggerated desquamation. Global RNA expression analysis revealed major changes in the expression of keratins, cornified envelope proteins, and cellular stress markers. These modifications of the keratinocyte genetic program are accompanied by changes in cell shape and disorganization of the actin cytoskeleton. These data indicate that loss of Lm332-mediated progenitor cell adhesion alters cell fate and disturbs epidermal homeostasis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tayem, Raneem UNSPECIFIED UNSPECIFIED UNSPECIFIED Niemann, Catherin UNSPECIFIED UNSPECIFIED UNSPECIFIED Pesch, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Morgner, Jessica UNSPECIFIED orcid.org/0000-0001-6374-8639 UNSPECIFIED Niessen, Carien M. UNSPECIFIED orcid.org/0000-0002-4892-9391 UNSPECIFIED Wickstrom, Sara A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Aumailley, Monique UNSPECIFIED orcid.org/0000-0002-4578-0966 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-587354
DOI:	10.1016/j.jid.2021.04.008
Journal or Publication Title:	J. Invest. Dermatol.
Volume:	141
Number:	11
Page Range:	S. 2602 - 2614
Date:	2021
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1523-1747
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ALPHA-6-BETA-4 INTEGRIN; EPIDERMOLYSIS-BULLOSA; TARGETED DISRUPTION; MOLECULAR-BASIS; HAIR FOLLICLE; LAMA3 GENE; KERATINOCYTES; ADHESION; BETA; ALPHA-3-BETA-1 Multiple languages Dermatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58735