Unnersjoe-Jess, David, Butt, Linus, Hoehne, Martin, Witasp, Anna, Kuehne, Lucas, Hoyer, Peter F., Patrakka, Jaakko, Brinkkoetter, Paul T., Wernerson, Annika, Schermer, Bernhard, Benzing, Thomas, Scott, Lena, Brismar, Hjalmar and Blom, Hans (2021). A fast and simple clearing and swelling protocol for 3D in-situ imaging of the kidney across scales. Kidney Int., 99 (4). S. 1010 - 1021. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1523-1755

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Abstract

In recent years, many light-microscopy protocols have been published for visualization of nanoscale structures in the kidney. These protocols present researchers with new tools to evaluate both foot process anatomy and effacement, as well as protein distributions in foot processes, the slit diaphragm and in the glomerular basement membrane. However, these protocols either involve the application of different complicated super resolution microscopes or lengthy sample preparation protocols. Here, we present a fast and simple, five-hour long procedure for three-dimensional visualization of kidney morphology on all length scales. The protocol combines optical clearing and tissue expansion concepts to produce a mild swelling, sufficient for resolving nanoscale structures using a conventional confocal microscope. We show that the protocol can be applied to visualize a wide variety of pathologic features in both mouse and human kidneys. Thus, our fast and simple protocol can be beneficial for conventional microscopic evaluation of kidney tissue integrity both in research and possibly in future clinical routines.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Unnersjoe-Jess, DavidUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Butt, LinusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoehne, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Witasp, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kuehne, LucasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoyer, Peter F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Patrakka, JaakkoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brinkkoetter, Paul T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wernerson, AnnikaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schermer, BernhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Benzing, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Scott, LenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brismar, HjalmarUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Blom, HansUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-591451
DOI: 10.1016/j.kint.2020.10.039
Journal or Publication Title: Kidney Int.
Volume: 99
Number: 4
Page Range: S. 1010 - 1021
Date: 2021
Publisher: ELSEVIER SCIENCE INC
Place of Publication: NEW YORK
ISSN: 1523-1755
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
MICROSCOPY; PATHOLOGY; TISSUEMultiple languages
Urology & NephrologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/59145

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