Schmitz, Katharina, Cox, Jan, Esser, Lea Marie, Voss, Martin, Sander, Katja, Loeffler, Antje, Hillebrand, Frank, Erkelenz, Steffen ORCID: 0000-0003-4763-1240, Schaal, Heiner ORCID: 0000-0002-1636-4365, Kaehne, Thilo, Klinker, Stefan, Zhang, Tao, Nagel-Steger, Luitgard, Willbold, Dieter, Seggewiss, Sabine, Schluetermann, David, Stork, Bjoern, Grimmler, Matthias ORCID: 0000-0003-2860-3391, Wesselborg, Sebastian and Peter, Christoph (2021). An essential role of the autophagy activating kinase ULK1 in snRNP biogenesis. Nucleic Acids Res., 49 (11). S. 6437 - 6456. OXFORD: OXFORD UNIV PRESS. ISSN 1362-4962

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Abstract

The biogenesis of small uridine-rich nuclear ribonucleoproteins (UsnRNPs) depends on the methylation of Sm proteins catalyzed by the methylosome and the subsequent action of the SMN complex, which assembles the heptameric Sm protein ring onto small nuclear RNAs (snRNAs). In this sophisticated process, the methylosome subunit pICIn (chloride conductance regulatory protein) is attributed to an exceptional key position as an 'assembly chaperone' by building up a stable precursor Sm protein ring structure. Here, we show that--apart from its autophagic role-the Ser/Thr kinase ULK1 (Uncoordinated [unc-51] Like Kinase 1) functions as a novel key regulator in UsnRNP biogenesis by phosphorylation of the C-terminus of pICIn. As a consequence, phosphorylated pICIn is no longer capable to hold up the precursor Sm ring structure. Consequently, inhibition of ULK1 results in a reduction of efficient UsnRNP core assembly. Thus ULK1, depending on its complex formation, exerts different functions in autophagy or snRNP biosynthesis.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Schmitz, KatharinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cox, JanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Esser, Lea MarieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Voss, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sander, KatjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Loeffler, AntjeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hillebrand, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Erkelenz, SteffenUNSPECIFIEDorcid.org/0000-0003-4763-1240UNSPECIFIED
Schaal, HeinerUNSPECIFIEDorcid.org/0000-0002-1636-4365UNSPECIFIED
Kaehne, ThiloUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Klinker, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhang, TaoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nagel-Steger, LuitgardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Willbold, DieterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Seggewiss, SabineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schluetermann, DavidUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stork, BjoernUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grimmler, MatthiasUNSPECIFIEDorcid.org/0000-0003-2860-3391UNSPECIFIED
Wesselborg, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Peter, ChristophUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-595460
DOI: 10.1093/nar/gkab452
Journal or Publication Title: Nucleic Acids Res.
Volume: 49
Number: 11
Page Range: S. 6437 - 6456
Date: 2021
Publisher: OXFORD UNIV PRESS
Place of Publication: OXFORD
ISSN: 1362-4962
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SMN COMPLEX; SEDIMENTATION-VELOCITY; PROTEINS; PHOSPHORYLATION; ULTRACENTRIFUGATION; METHYLOSOME; MACHINERY; PRMT5; ICLN; 6SMultiple languages
Biochemistry & Molecular BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/59546

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