Universität zu Köln

Kraehling, Verena, Halwe, Sandro, Rohde, Cornelius, Becker, Dirk, Berghoefer, Susanne, Dahlke, Christine, Eickmann, Markus, Ercanoglu, Meryem S., Gieselmann, Lutz, Herwig, Astrid, Kupke, Alexandra, Mueller, Helena, Neubauer-Raedel, Petra, Klein, Florian, Keller, Christian and Becker, Stephan (2021). Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies. J. Immunol. Methods, 490. AMSTERDAM: ELSEVIER. ISSN 1872-7905

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Abstract

The current Severe acute respiratory syndrome related coronavirus 2 (SARS-CoV-2) pandemic is a public health emergency of international concern. Sensitive and precise diagnostic tools are urgently needed. In this study, we developed a SARS-CoV-2 spike (S1) protein enzyme-linked immunosorbent assay (ELISA) to detect SARS-CoV-2-specific antibodies. The SARS-CoV-2 S1 ELISA was found to be specific [97.8% (95% CI, 96.7% - 98.5%)], reproducible and precise (intra-assay coefficient of variability (CV) 5.3%, inter-assay CV 7.9%). A standard curve and the interpolation of arbitrary ELISA units per milliliter served to reduce the variability between different tests and operators. Cross-reactivity to other human coronaviruses was addressed by using sera positive for MERS-CoV- and hCoV HKU1-specific antibodies. Monitoring antibody development in various samples of twenty-three and single samples of twenty-nine coronavirus disease 2019 (COVID-19) patients revealed seroconversion and neutralizing antibodies against authentic SARS-CoV-2 in all cases. The comparison of the SARS-CoV-2 (S1) ELISA with a commercially available assay showed a better sensitivity for the in-house ELISA. The results demonstrate a high reproducibility, specificity and sensitivity of the newly developed ELISA, which is suitable for the detection of SARS-CoV-2 S1 protein-specific antibody responses.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kraehling, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Halwe, Sandro UNSPECIFIED UNSPECIFIED UNSPECIFIED Rohde, Cornelius UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED Berghoefer, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Dahlke, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Eickmann, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Ercanoglu, Meryem S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gieselmann, Lutz UNSPECIFIED UNSPECIFIED UNSPECIFIED Herwig, Astrid UNSPECIFIED UNSPECIFIED UNSPECIFIED Kupke, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Helena UNSPECIFIED UNSPECIFIED UNSPECIFIED Neubauer-Raedel, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Keller, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-596829
DOI:	10.1016/j.jim.2021.112958
Journal or Publication Title:	J. Immunol. Methods
Volume:	490
Date:	2021
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1872-7905
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Biochemical Research Methods; Immunology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59682