Universität zu Köln

Hoppel, Florian, Garcia-Souza, Luiz Felipe, Kantner-Rumplmair, Wilhelm, Burtscher, Martin ORCID: 0000-0002-5232-3632, Gnaiger, Erich ORCID: 0000-0003-3647-5895, Pesta, Dominik ORCID: 0000-0002-5089-3586 and Calabria, Elisa ORCID: 0000-0001-6557-0379 (2021). Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies. Cells, 10 (8). BASEL: MDPI. ISSN 2073-4409

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Abstract

Human blood cells may offer a minimally invasive strategy to study systemic alterations of mitochondrial function. Here we tested the reliability of a protocol designed to study mitochondrial respiratory control in human platelets (PLTs) in field studies, using high-resolution respirometry (HRR). Several factors may trigger PLT aggregation during the assay, altering the homogeneity of the cell suspension and distorting the number of cells added to the two chambers (A, B) of the Oroboros Oxygraph-2k (O2k). Thus, inter-chamber variability ( increment ab) was calculated by normalizing oxygen consumption to chamber volume (J(O2)) or to a specific respiratory control state (flux control ratio, FCR) as a reliable parameter of experimental quality. The method's reliability was tested by comparing the increment ab of laboratory-performed experiments (LAB, N = 9) to those of an ultramarathon field study (three sampling time-points: before competition (PRE, N = 7), immediately after (POST, N = 10) and 24 h after competition (REC; N = 10)). Our results show that increment ab J(O2) changed PRE-POST, but also for LAB-POST and LAB-REC, while all increment ab FCR remained unchanged. Thus, we conclude that our method is reliable for assessing PLT mitochondrial function in LAB and field studies and after systemic stress conditions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hoppel, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Garcia-Souza, Luiz Felipe UNSPECIFIED UNSPECIFIED UNSPECIFIED Kantner-Rumplmair, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Burtscher, Martin UNSPECIFIED orcid.org/0000-0002-5232-3632 UNSPECIFIED Gnaiger, Erich UNSPECIFIED orcid.org/0000-0003-3647-5895 UNSPECIFIED Pesta, Dominik UNSPECIFIED orcid.org/0000-0002-5089-3586 UNSPECIFIED Calabria, Elisa UNSPECIFIED orcid.org/0000-0001-6557-0379 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-599338
DOI:	10.3390/cells10082088
Journal or Publication Title:	Cells
Volume:	10
Number:	8
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2073-4409
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PERMEABILIZED FIBERS; OXPHOS PROTOCOLS; SMALL BIOPSIES; HUMAN-CELLS; SKELETAL; RESPIRATION; MUSCLE; RESPIROMETRY; DYSFUNCTION; METABOLISM Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59933