Aufsatz
Artikel (Publikationen im Open Access gefördert durch die UB)
Microsatellites from Fosterella christophii (Bromeliaceae) by de novo transcriptome sequencing on the Pacific Biosciences RS platform
Zusammenfassung
• Premise of the study: Microsatellite markers were developed in Fosterella christophii (Bromeliaceae) to investigate the genetic diversity and population structure within the F. micrantha group, comprising F. christophii, F. micrantha, and F. villosula. • Methods and Results: Full-length cDNAs were isolated from F. christophii and sequenced on a Pacific Biosciences RS platform. A total of 1590 high-quality consensus isoforms were assembled into 971 unigenes containing 421 perfect microsatellites. Thirty primer sets were designed, of which 13 revealed a high level of polymorphism in three populations of F. christophii, with four to nine alleles per locus. Each of these 13 loci cross-amplified in the closely related species F. micrantha and F. villosula, with one to six and one to 11 alleles per locus, respectively. • Conclusions: The new markers are promising tools to study the population genetics of F. christophii and to discover species boundaries within the F. micrantha group.
Zitierform
In: Applications in plant sciences. - Washington, DC : BioOne, 2016, 4 (1):1500084, 1-4Förderhinweis
Gefördert durch den Publikationsfonds der Universität KasselSammlung(en)
Publikationen (Fachgebiet Botanik (Systematik und Morphologie))Artikel (Publikationen im Open Access gefördert durch die UB)
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@article{urn:nbn:de:hebis:34-2016020549834,
author={Wöhrmann, Tina and Huettel, Bruno and Wagner, Natascha and Weising, Kurt},
title={Microsatellites from Fosterella christophii (Bromeliaceae) by de novo transcriptome sequencing on the Pacific Biosciences RS platform},
year={2016}
}
0500 Oax 0501 Text $btxt$2rdacontent 0502 Computermedien $bc$2rdacarrier 1100 2016$n2016 1500 1/eng 2050 ##0##urn:nbn:de:hebis:34-2016020549834 3000 Wöhrmann, Tina 3010 Huettel, Bruno 3010 Wagner, Natascha 3010 Weising, Kurt 4000 Microsatellites from Fosterella christophii (Bromeliaceae) by de novo transcriptome sequencing on the Pacific Biosciences RS platform / Wöhrmann, Tina 4030 4060 Online-Ressource 4085 ##0##=u http://nbn-resolving.de/urn:nbn:de:hebis:34-2016020549834=x R 4204 \$dAufsatz 4170 7136 ##0##urn:nbn:de:hebis:34-2016020549834
2016-02-05T10:26:05Z 2016-02-05T10:26:05Z 2016 2168-0450 urn:nbn:de:hebis:34-2016020549834 http://hdl.handle.net/123456789/2016020549834 Gefördert durch den Publikationsfonds der Universität Kassel eng Urheberrechtlich geschützt https://rightsstatements.org/page/InC/1.0/ 580 Microsatellites from Fosterella christophii (Bromeliaceae) by de novo transcriptome sequencing on the Pacific Biosciences RS platform Aufsatz • Premise of the study: Microsatellite markers were developed in Fosterella christophii (Bromeliaceae) to investigate the genetic diversity and population structure within the F. micrantha group, comprising F. christophii, F. micrantha, and F. villosula. • Methods and Results: Full-length cDNAs were isolated from F. christophii and sequenced on a Pacific Biosciences RS platform. A total of 1590 high-quality consensus isoforms were assembled into 971 unigenes containing 421 perfect microsatellites. Thirty primer sets were designed, of which 13 revealed a high level of polymorphism in three populations of F. christophii, with four to nine alleles per locus. Each of these 13 loci cross-amplified in the closely related species F. micrantha and F. villosula, with one to six and one to 11 alleles per locus, respectively. • Conclusions: The new markers are promising tools to study the population genetics of F. christophii and to discover species boundaries within the F. micrantha group. open access In: Applications in plant sciences. - Washington, DC : BioOne, 2016, 4 (1):1500084, 1-4 Wöhrmann, Tina Huettel, Bruno Wagner, Natascha Weising, Kurt doi:10.3732/apps.1500084
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