Climate change and glacial retreat are changing the runoff behavior of Alpine springs and streams. For example, in the extremely dry and hot summer of 2018, many springs used for drinking water supply lost up to 50 percent of their average discharge; a few springs have even run dry. In order to ensure drinking water supply in the future, springs featuring large and constantly sufficient discharge rates will have to be identified and tapped. A case study was undertaken at the Tuxbachquelle because catchment area and temporal variation of physicochemical and hydrochemical properties were previously unknown. Tracer tests with uranine proved a hydraulic connection between this karst spring and a stream a few kilometers uphill. At low runoff, uranine needed about 4½ hours from the sink to the spring, whereas at high runoff more than four days was required. It became evident that discharge, electrical conductivity, temperature, and turbidity of the Tuxbachquelle respond within a few hours to precipitation events. The water quality and an examination of the water balance resulted in a significantly larger catchment area. It is assumed that widely karstified calcite marble subterraneously drains a considerable part of the Tuxertal (Tux Valley), including some active rock glaciers.