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A tightly regulated and adjustable CRISPR-dCas9 based AND gate in yeast

Hofmann, Anja ; Falk, Johannes ; Prangemeier, Tim ; Happel, Dominic ; Köber, Adrian ; Christmann, Andreas ; Koeppl, Heinz ; Kolmar, Harald (2019)
A tightly regulated and adjustable CRISPR-dCas9 based AND gate in yeast.
In: Nucleic Acids Research, 2018, 47 (1)
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: A tightly regulated and adjustable CRISPR-dCas9 based AND gate in yeast
Language: English
Date: 1 February 2019
Place of Publication: Darmstadt
Year of primary publication: 2018
Publisher: Oxford Academic
Journal or Publication Title: Nucleic Acids Research
Volume of the journal: 47
Issue Number: 1
Corresponding Links:
Origin: Secondary publication via sponsored Golden Open Access
Abstract:

The robust and precise on and off switching of one or more genes of interest, followed by expression or repression is essential for many biological circuits as well as for industrial applications. However, many regulated systems published to date influence the viability of the host cell, show high basal expression or enable only the overexpression of the target gene without the possibility of fine regulation. Herein, we describe an AND gate designed to overcome these limitations by combining the advantages of three well established systems, namely the scaffold RNA CRISPR/dCas9 platform that is controlled by Gal10 as a natural and by LexA-ER-AD as heterologous transcription factor. We hence developed a predictable and modular, versatile expression control system. The selection of a reporter gene set up combining a gene of interest (GOI) with a fluorophore by the ribosomal skipping T2A sequence allows to adapt the system to any gene of interest without losing reporter function. In order to obtain a better understanding of the underlying principles and the functioning of our system, we backed our experimental findings with the development of a mathematical model and single-cell analysis.

Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-84327
Classification DDC: 500 Science and mathematics > 530 Physics
Divisions: 05 Department of Physics > Institute for condensed matter physics (2021 merged in Institute for Condensed Matter Physics)
Date Deposited: 01 Feb 2019 09:32
Last Modified: 10 Nov 2023 10:47
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/8432
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