- AutorIn
- Evgeni Sperling Technische Universität Dresden
- Titel
- Dynamisches Verhalten teilgestreckter DNA-Moleküle in submikrofluidischen Kanälen
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-364127
- Datum der Einreichung
- 03.05.2019
- Datum der Verteidigung
- 21.10.2019
- Abstract (EN)
- The investigation of the physical properties of deoxyribonucleic acid under confinement is an essential step for the all-embracing understanding of the replication and transcription in living cells as well as for the development of the biomimetic nanotechnology. The following report addresses the measure-ment and interpretation of the intramolecular diffusion along stretched λ-DNA-molecules. This work comprises the fabrication of submicroscopic channels via softlithography, the integration of the chan-nels in an experimental setup with a fluorescence microscope and a source-measurement unit, and the experiments with the DNA-stretching in electrical field. The important results are the development of a measuring assembly with stable, softlithographic structures in Ormostamp, the direct imaging and measurement of the intermolecular diffusion along stretched DNA-molecules in channels with cross sections down to 100 x 300 nm2, and the qualitative and quantitative analysis on the basis of models of polymer physics.
- Verweis
- DNA under confinement and the use of DNA as confinement
Link: http://diffusion.uni-leipzig.de/pdf/volume23/TP2_SFG-Abschlussbericht.pdf - Freie Schlagwörter (DE)
- DNA-Stretching, Softlithographie, Mikrofluidik
- Freie Schlagwörter (EN)
- DNA stretching, soft lithography, microfluidics
- Klassifikation (DDC)
- 540
- Klassifikation (RVK)
- UF 4270
- GutachterIn
- Prof. Dr.-Ing. habil. Gerald Gerlach
- Prof. Dr. rer. nat. et Ing. Michael Mertig
- Den akademischen Grad verleihende / prüfende Institution
- Technische Universität Dresden, Dresden
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-364127
- Veröffentlichungsdatum Qucosa
- 03.12.2019
- Dokumenttyp
- Dissertation
- Sprache des Dokumentes
- Deutsch
- Lizenz / Rechtehinweis