- AutorIn
- René Thieme
- Susanne Kurz
- Marlen Kolb
- Tewodros Debebe
- Susanne Holtze
- Michaela Morhart
- Klaus Huse
- Karol Szafranski
- Matthias Platzer
- Thomas B. Hildebrandt
- Gerd Birkenmeier
- Titel
- Analysis of alpha-2 macroglobulin from the long-lived and cancer-resistant naked mole-rat and human plasma
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa-175598
- Quellenangabe
- PLoS one 10(6): e0130470
- Erstveröffentlichung
- 2015
- Abstract (EN)
- Background: The naked mole-rat (NMR) is a long-lived and cancer resistant species. Identification of potential anti-cancer and age related mechanisms is of great interest and makes this species eminent to investigate anti-cancer strategies and understand aging mechanisms. Since it is known that the NMR expresses higher liver mRNA-levels of alpha 2-macroglobulin than mice, nothing is known about its structure, functionality or expression level in the NMR compared to the human A2M. Results: Here we show a comprehensive analysis of NMR- and human plasma-A2M, showing a different prediction in glycosylation of NMR-A2M, which results in a higher molecular weight compared to human A2M. Additionally, we found a higher concentration of A2M (8.3±0.44 mg/mL vs. and 4.4±0.20 mg/mL) and a lower total plasma protein content (38.7±1.79 mg/mL vs. 61.7±3.20 mg/mL) in NMR compared to human. NMR-A2M can be transformed by methylamine and trypsin resulting in a conformational change similar to human A2M. NMRA2M is detectable by a polyclonal antibody against human A2M. Determination of tryptic and anti-tryptic activity of NMR and human plasma revealed a higher anti-tryptic activity of the NMR plasma. On the other hand, less proteolytic activity was found in NMR plasma compared to human plasma.
- Freie Schlagwörter (DE)
- Krebs, Nacktmull, mRNA-Spiegel, Gene
- Freie Schlagwörter (EN)
- Cancer, naked mole-rat, mRNA-level, gene
- Klassifikation (DDC)
- 610
- Herausgeber (Institution)
- Universität Leipzig
- Leibniz-Institut für Zoo- und Wildtierforschung (IZW) im Forschungsverbund Berlin e.V.
- Leibniz-Institut für Altersforschung - Fritz-Lipmann-Institut e.V. (FLI)
- PLoS
- Förder- / Projektangaben
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa-175598
- Veröffentlichungsdatum Qucosa
- 27.07.2015
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch